NO在大鼠脑缺血再灌注中对局部脑血流量和脑水肿作用的研究

The Effect of Nitric Oxide on Brain Edema and Regional Cerebral Blood Flow after Brain Ischemia/Reperfusion in the Wistar Rats

目的：研究ＮＯ在脑缺血再灌注中对局部脑血流量和脑水肿的影响，为临床改进脑缺血的治疗提供新思路。方法：采用拴线法大鼠ＭＣＡ缺血再灌注模型，利用外源性ＮＯ前体（Ｌ－ａｒｇ）和ＮＯＳ抑制剂（Ｌ－ＮＡＭＥ）。将４２只Ｗｉｓｔａｒ大鼠随机分组，在ｒ－ＣＢＦ组和脑水肿两大组中分别设有：缺血再灌注盐水组，再灌注Ｌ－ａｒｇ组，再灌注Ｌ－ＮＡＭＥ组。结果：与盐水组比较，Ｌ－ａｒｇ组ｒ－ＣＢＦ明显升高（Ｐ＜０．０５），脑水肿显著减轻（Ｐ＜０．０５）；Ｌ－ＮＡＭＥ组ｒ－ＣＢＦ明显降低（Ｐ＜０．０５），脑水肿显著加重（Ｐ＜０．０５）。结论：ＮＯ对缺血再灌注脑组织有保护性作用。

Objective: We studied the effects of Nitric Oxide (NO) on brain edema and r CBF after ischemia/reperfusion. Our purpose was to produce a new thought for improving the treatment of brain ischemia.Methods:We used Wistar rat MCA ischemia/reperfusion model, which was made by blocking the external cervical artery with thread for obstruction. The level of NO was modulated by L arginine, an exogenous precursor of NO and Nitric oxide synthase (NOS) inhibitor (L NAME).Forty two Wister rats were randomly assinged to groups.First, the r CBF group and brain edema group were determined.Then both r CBF and brain edema groups were further divided into three groups, namely:L arg, L NAME,and the control.Results:r CBF increased significantly (P<0.05) and brain edema decreased obviously (P<0.05) in L arg group as compared with those of the control. r CBF decreased sharply (P<0.05) and brain edema increased significantly (P<0.05) in L NAME group in comparison with those of the control.Conclusions:NO was protective for brain tissue in ischemia/ reperfusion.