加温联合六亚甲基二乙酰胺对MEC-1细胞的作用

Effects of hyperthermia combined with hexamethylene bisacetamide on mucoepidermoid carcinoma MEC-1 cells

目的：研究加温联合六亚甲基二乙酰胺（ＨＭＢＡ）对人粘液表皮样癌ＭＥＣ－１细胞的作用。方法：采用ＭＴＴ法及软琼脂克隆形成法观察加温联合ＨＭＢＡ对ＭＥＣ－１细胞的作用；并用免疫组化法检测加温及联合ＨＭＢＡ对多药耐药（ＭＤＲ）表达蛋白（Ｐ－ｇｐ）的影响。结果：加温联合ＨＭＢＡ对ＭＥＣ－１细胞有协同抑制作用。免疫组化染色显示，对照组及单用药组细胞Ｐ－ｇｐ中度表达；加温组细胞Ｐ－ｇｐ不表达；联合组为低度表达。结论：加温联合ＨＭＢＡ对粘液表皮样癌的治疗可能有一定价值。

Objective:To study the effects of hyperthermia combined with hexamethylene bisacetamide(HMBA) on mucoepidermoid carcinoma MEC-1 cells. Methods:MEC-1 cells were treated with HMBA at various concentrations ,or hyperthermia at 42..5℃ for 1h,or of the combination of both.The inhibitory effects were investigated by MTT assay and colonogenic assey respectively.Expression of MDR protin P-gp was measured by immuno-histochemical staining. Results:1)hyperthermia could intensify the effects of HMBA; 2)MEC-1 cells expressed P-gp protein at a medium level , HMBA did not show effects on the expression ,but hyperthermia alone or with HMBA suppressed P-gp expression. Conclusion:Hyperthermia and HMBA may be potential in treating mucoepidermoid carcinoma.