唾液对体外培养NIH-3T3及MEC-1细胞粘附的影响

Effect of human saliva on adhesion of NIH 3T3 and MEC-1 cells in vitro

目的：探讨人唾液对正常及肿瘤细胞在体外粘附有无不同影响。方法：接种ＮＩＨ—３Ｔ３及ＭＥＣ－１细胞在预先涂有或未涂唾液的２４孔培养板上，用细胞计数法分别在接种后２、４、６和８ｈ计数贴壁细胞。结果：ＮＩＨ－３Ｔ３细胞及ＭＥＣ－１细胞在涂有唾液的塑料底物上在２、４、６、和８ｈ其贴壁抑制率分别为３５％、４１％、２６％、３４％和１０％、９％、８％、４％。结论：人全唾液能抑制体外培养的ＮＩＨ－３Ｔ３细胞的粘附，但对ＭＥＣ－１细胞的影响不大。

Objective : To compare the effects of human saliva on adhesion of normal and cancer cells. Methods : NIH-3T3 and MEC-1 cells, 2×10 8 cells/L, were seeded in 24 wells culture plates pre coated with or without human whole saliva and the numbers of adhered cells were determined by cell counting at 2,4,6,and 8 h after seeding. Results: The inhibition rates of attached NlH-3T3 cells by saliva at 2,4,6 and 8 h were 35%、41%、26% 34% , and those of MEC-1 were 10%、9%、8% and 4%,respectively. Conclusion: Human saliva may inhibit adhesion of NIH-3T3 in vitro , but it had little effect on MEC-1 cells.