牙周炎患者全唾液中蛋白质的等电聚焦分析

Isoelectric focusing analysis of whole salivary proteins in the patients with periodontitis

目的：对正常人及牙周炎患者全唾液进行等电聚焦分析，初步观察其蛋白质分布变化。方法：采集３３例正常人，２６例牙周炎患者全唾液，运用聚丙烯酰胺凝胶等电聚焦方法观察其蛋白质等电点分布。结果：正常人全唾液中蛋白质等电点区带为３０条，牙周炎组全唾液中蛋白质区带为３９条，其中ｐＨ７．０以上的蛋白质区带较正常人组明显增多。牙周炎组全唾液碱性蛋白质区带数目增多。结论：牙周炎组全唾液碱性蛋白质区带数目增多，可能与其含有较多的碱性氨基酸有关，表明牙周炎状态下，唾液分泌蛋白质种类的增加，唾液成份代谢产物或牙周致病菌的产物发生改变。

Objiectives: To compare the differences of proteins in whole saliva between periodontitis patients and healthy controls.Methods: Saliva was collected from 33 healthy and 26 periodontitis individuals respectively, and analysed with isoelectric focusing in polyacrylamide gels.Results: There were 39 protein bands in the periodontitis group whereas only 30 in the control, and the PIs of the 4 of the 9 extra bands were higher than 7.00. Conclusions: In the saliva of periodontitis individuals there are more basic proteins which may contain more basic amino acids.It indicates the increase the types of salivary proteins and the change of metabolite of salivary component and germer under periodontitis condition.