CD_4T细胞活化与白细胞介素5释放在过敏性哮喘发病中的作用

Activation of CD 4 T lymphocyte and release of interleukin 5 in airway from patients with atopic asthmatics

目的了解特异性过敏原刺激下ＣＤ４Ｔ细胞活化以及白细胞介素５（ＩＬ５）释放在过敏性哮喘发病中作用。方法对１２例过敏性哮喘（ＡＡ组）、９例过敏性非哮喘（ＡＮ组）患者以及１０例正常对照者（Ｎ组）用过敏原屋尘螨提取液（ＨＤＭ）进行全肺吸入激发，测定激发前后支气管肺泡灌洗（ＢＡＬ）液和周围血单个核细胞（ＰＢＭＣ）的ＣＤ４ＣＤ２５细胞、嗜酸粒细胞（ＥＯＳ）、ＩＬ５、嗜酸细胞阳离子蛋白（ＥＣＰ）水平。结果ＡＡ组迟发气道反应（ＬＡＲ）发生率明显高于ＡＮ组，差异有非常显著意义（Ｐ＜００１），此种差异与ＢＡＬ液中的ＥＯＳ计数（ｒ＝０５３９，Ｐ＜００５）、ＣＤ４ＣＤ２５细胞、ＩＬ５及ＥＣＰ水平相关。结论ＣＤ４Ｔ细胞活化与哮喘状态有关，也与过敏状态有关；特异过敏原刺激是过敏性哮喘病人ＣＤ４Ｔ细胞活化的重要原因；ＩＬ５是ＥＯＳ选择性活化因子。

Objective To evaluate the action of CD 4T cell and release of interleukin 5 in airway from patients with atopic asthma by the allergen specific stimulation. Methods Twelve atopic asthmatics (AA), 9 atopic non asthmatics (AN), and 10 normal controls(N) underwent whole lung inhalation challenge with house dust mite allergen (HDM) extract.The Levels of CD 4CD 25 T lymphocytes, eosinophils(EOS), interleukin 5 (IL 5) and eosinophil cationic protein (ECP) in bronchoalveolar lavage (BAL) and peripheral blood mononuclear cells (PBMC) were investigated with and without HDM challenge.Results AA differed from AN in having late airway reactions (LAR) after HDM inhalation ( P <0.01), which was correlated with an percentage of BAL eosinophils, CD 4CD 25 T cell IL 5 production and ECP release. Conclusion These findings suggest that the activation of CD 4T lymphocytes is correlated with both asthmatic and atopic status. The allergen specific stimulation is believed to play a major role in CD 4 T cell activation in atopic asthma. IL 5 is selective cytokine for EOS activation and modulating local eosinophil recruitment and activation in airway