产表活石油微生物菌株的鉴定与离子束诱变改良

Identification and mutation breeding of a surfactant-producing microorganism strain by ion beam

对自行分离的石油微生物经鉴定为地衣芽孢杆菌,发酵产物的成分通过薄层层析和红外光谱分析认为,发酵产物的有效成分是环脂肽类物质。以H菌为出发菌株,用15keVN+以0～4×1015ions·cm-2的剂量进行了辐照。存活曲线呈"马鞍型",与前人的结果相同,以2.5×1015ions·cm-2为拐点,此时存活率为23.3%,正突变率为36.7%。单菌落发酵实验表明,随着剂量的增大,各处理的发酵液的表面张力值有减小的趋势,以2.5×1015ions·cm-2剂量的表面张力为最小。最终初筛出5个突变菌株,并经遗传稳定性分析,获得两个表现较为稳定的优良菌株M314和M325。

The MEOR related microorganism strain DG-H07 was indentified as the fermentation components of were analyzed with TLC and NF-IR. It could be concluded that the effective component is cyclic lipopeptide. The H strain was irradiated with 15keV N＋ at the dose of 0-4×10^15ions·cm-2. The ＂cradle model＂ survival curve , ac- cords with the previous studies. The turning point is the dose of 2.5×10^15ions·cm-2, with the survival rate of 23.3% and the positive mutation rate of 36.7%. The single clony fermentation experiments shows that with the rising of dose treated, the surface tension of the fermentation broth of each group had the tendency to decrease, while the group treated at the dose of 2,5×10^15ions·cm-2 had the lowest average surface tension. Uhimately 5 mutants were screened from the elonies and 2 trains, M314 and M325 with a stable expression after the evaluation of 5 genera- tions.