稀土对黄连愈伤组织生长的影响

EFFECT OF RARE EARTH ELEMENTS ON CALLUS GROWTH IN COPTIS CHINENSIS

目的与方法：探讨稀土对药用植物细胞组织培养的作用和意义。以药用植物黄连为材料，研究不同浓度的稀土对愈伤组织鲜重增加的影响。将从黄连外植体上诱导出的愈伤组织接种于添加不同浓度稀土Ｙｂ３＋和Ｅｕ３＋的６７Ｖ培养基上。培养一段时间后，精密称量鲜重，研究不同浓度稀土对黄连愈伤组织生长的影响和对转入无稀土培养基中的愈伤组织后续生长的影响。结果：０．１ｍｇ·Ｌ－１Ｙｂ３＋和１ｍｇ·Ｌ－１Ｅｕ３＋促进愈伤组织的生长，高浓度稀土抑制愈伤组织的生长。在不含稀土的培养基上的继代培养中，来源于稀土处理的愈伤组织鲜重增加值均比对照低。结论：低浓度的稀土对黄连愈伤组织的生长具有促进作用。

Objective and methods: To study the effect of rare earth elements on cell and tissue cultures of the medicinal plants. Using Coptis chinensis as plant material, the effect of the rare earth elements Yb 3+ and Eu 3+ at various concentrations on the callus growth were investigated. The calli, induced from explants in Coptis chinens , were inoculated on 67 V media containing various concentration of Yb 3+ and Eu 3+ . After a period of culture, calli were weighed precisely. We studied the effect of Yb 3+ and Eu 3+ at various concentrations on the callus growth and the continuing growth of calli transferred on the medium free of rare earth elements. Results: 0.1 mg·L -1 Yb 3+ and 1 mg·L -1 Eu 3+ stimulated the callus growth, but higher concentrations inhibited the growth. The callus increment of fresh weight was lower than the control when the calli, derived from the media containing various concentrations of Yb 3+ and Eu 3+ , were transferred and cultured on the medium free of rare earth. Conclusion: The callus growth was stimulated by the lower concentration of rare earth elements. The lower and higher concentration of Yb 3+ and Eu 3+ was deleterious to the continuing growth of the calli transferred on the medium free of rare earth.