骨巨细胞瘤中的多核巨细胞体外骨吸收研究

IN VITRO BONE RESORPTION INDUCED BY CULTURED MULTINUCLEATED GIANT CELLS ISOLATED FROM GIANT CELL TUMOUR

目的：比较破骨细胞与骨巨细胞瘤中多核巨细胞的特点，明确后者的性质和来源。方法：用倒置相差显微镜观察体外培养的多核巨细胞的一般形态及降钙素对它的影响；用骨片与多核巨细胞共同培养法观察多核巨细胞的体外骨吸收功能，用扫描电镜观察骨吸收陷窝，Ｇｏｍｏｒｉ染色观察多核巨细胞的酸性磷酸酶活性。结果：倒置相差显微镜下可见多核巨细胞胞核较多（２０个以上），胞浆周边不规则，有伪足样突起；胞浆内可见较多大小不等的空泡；降钙素（１００μｇ·Ｌ－１）可抑制多核巨细胞的伪足样运动；多核巨细胞与灭活的骨片共同培养时可见骨吸收陷窝形成，扫描电镜下可见吸收陷窝底面有原纤维；Ｇｏｍｏｒｉ染色时可见多核巨细胞的酸性磷酸酶呈阳性。结论：证实了多核巨细胞具有破骨细胞的形态特征与骨吸收功能。

Objective: To compare the characteristics of cultured multinucleated giant cells(MGC)isolated from giant cell tumour of bone (GCT) and the osteoclast, to find out the origin of MGC. Methods: Morphologic features of MGC and reactions induced by calcitonin were observed with inverted phase contrast microscope. The function of bone resorption by MGC was examined with coculture of bone slice and MGC. The resorption lacunae were examined with a scanning electron microscope (SEM). The activity of acid phosphatase in MGC was observed by Gomori stain. Results: MGCs showed multinuclei (more than 20 nuclei), irriegular peripheral cytoplasm with pseudopodial projections, vacuoles were seen in the cytoplasm. the pseudopodial movement could be inhibited by calcitonin (100 μg·L -1 ). Lacuna formation could be found in cocultured specimens. There were fibrils on the walls of lacunae. The MGCs demonstrated positive reaction with Gomori stain. Conclusion: The results demonstrated that MGCs had the same morphological features and the bone resorption ability as the osteoclasts. Therefore, the MGCs might be originated from the osteoclasts of stem cell in bone marrow.