北极狐SRAP分子标记的多态性初步研究

Arctic fox SRAP marker polymorphism of the preliminary study

相关序列多态性(SRAP)是近年来发展起来的一种新型分子标记技术,具有稳定、简便、中等产率、高共显性等优点。实验利用北极狐的基因组为模版,首次把SRAP方法引入到哺乳动物中进行分析,对北极狐SRAP反应条件进行了优化,确立了北极狐SRAP-PCR的反应条件是:94℃预变性5min,94℃1min,35℃1min,72℃2min,5个循环;94℃变性1min,94℃1min,55℃1min(根据不同的引物设定),72℃1min,35个循环,72℃延伸10min,最后产物用6%的非变性的聚丙烯酰胺凝胶分离,得到的结果清晰、稳定、多态性高,条带可以用在后续对北极狐的遗传多样的分析和品种鉴定上。

Related sequence polymorphism （SRAP） in recent years was a new type of molecular marker technology, with a stable, simple, middle-yield, high-codominant advantages. The use of the Arctic fox＇s genome as a template was the first time for the SRAP approach to the introduction of mammals. The analysis of the Arctic fox SRAP reaction conditions was optimized to establish. The Arctic fox SRAP-PCR reaction conditions were： 94 ℃ predegeneration 5rain, 94 ℃ 1min, 35 ℃ 1min, 72 ℃ 2min, 5 cycles; 94 ℃ degeneration lmin, 94 ℃ 1min, 55 ℃1min （depending on the primer set）, 72 ℃ 1min, 35 cycles, 72 ℃ extension 10min. The final product was 6% of the non-denaturing polyacrylamide gel separation. The results are clear and stable. The high-band polymorphism can be used in a variety of genetic analysis and identification of the species of the Arctic fox.