含双T切换接口的胶束电动色谱-毛细管区带电泳二维电泳芯片上混合氨基酸的分离富集

Separation and Enrichment of Amino Acids on Micellar Electrokinetic Chromatography-Capillary Zone Electrophoresis Two-dimensional Microfluidic Chip with Double T Switching Interface

基于时间顺序设计提出了含双T切换接口的胶束电动色谱(MEKC)和毛细管区带电泳(CZE)的二维芯片,构建了相应的芯片分析测试系统。基于FITC标记的氨基酸样品的一维MEKC和CZE实验结果,对二维进样时间、二维分离启动时间等二维芯片电泳关键操作参数进行了优化。采用所构建的MEKC-CZE二维芯片电泳分析系统对精氨酸、赖氨酸、组氨酸、苯丙氨酸和酪氨酸的混合样品进行了二维芯片电泳分离分析,计算得到两种分离模式的正交性为56.0%。

Micellar electrokinetic chromatography-capillary zone electrophoresis（MEKC-CZE） two-dimensional microfluidic chip with double T switching interface based on time sequences separation was designed and the relative analytical system was established.The mixed amino acids labeled by FITC were taken as samples and separated by this analytical system on the designed MEKC-CZE chip.Several conditions effected on the separation,such as startup time and injection time of the second dimension,were optimized.The separation of amino acids,including L-Arginine,L-Lysine,L-Histidine,L-Phenylalanine and L-Tyrosine,was carried out on the setup microchip.It was illustrated that complete separation of components was realized.The calculated orthogonality of MEKC and CZE was 56.0%.