流动注射后化学发光法测定阿米卡星

Flow Injection Post-Chemiluminescence Analysis of Amikacin

过氧化单硫酸盐具有很强的氧化性,本实验发现,在pH=11.5的条件下,过氧化单硫酸盐(PMS)可以氧化鲁米诺(Luminol)产生瞬时化学发光,发光结束后注入阿米卡星又会出现很强的慢速后化学发光。结合流动注射的方法,利用此体系建立了流动注射后化学发光测定阿米卡星的新方法,并对其后化学发光反应的动力学性质、化学发光光谱进行了研究,讨论了可能的反应机理。阿米卡星浓度在4.0×10-5～8.0×10-2g/L范围内与发光强度呈良好的线性关系,检出限(3σ)为1×10-5g/L,相对标准偏差为2.9%。本方法具有选择性好、操作简单、分析速度快和重复性好等优点,已成功用于尿液中阿米卡星含量的测定,结果令人满意。

In basic medium of pH=11.5,peroxymonosulfate（PMS）,a strong oxidant,can oxidize the luminol to produce chemiluminescence,and after the injection of amikacin,the luminescence rate becomes slow and strong post-chemiluminescence（PCL）.Based on this,a new CL method was developed for the determination of amikacin using flow injection chemiluminescence analysis.A possible mechanism for the CL was discussed via the studies of kinetic characteristics,the CL spectra and UV spectrum.The linear range was 4.0×10^-5-8.0×10^-2 g/L with a detection limit of 1×10^-5 g/L for amikacin and the relative standard deviation was 2.9%（c=4.0×10^-6 g/mL,n=11）.The proposed method was selective,simple,rapid and reproducible,and has been successfully applied to the analysis of human urine samples with satisfactory results.