17-β-雌二醇对原代人脐静脉内皮细胞一氧化氮生成的影响

17 β-estradiol enhancing the production of nitric oxide from the primary cultures of human umbilical vein endothelial cells

目的观察１７β雌二醇对原代人脐静脉内皮细胞（ＨＵＶＥＣ）一氧化氮（ＮＯ）生成的影响。方法采用胰蛋白酶灌流消化法培养原代ＨＵＶＥＣ，以原代ＨＵＶＥＣ为对象，观察不同浓度的１７β雌二醇（１００ｎｍｏｌ／Ｌ，３０ｎｍｏｌ／Ｌ，１０ｎｍｏｌ／Ｌ，１ｎｍｏｌ／Ｌ，０．１ｎｍｏｌ／Ｌ）作用于原代ＨＵＶＥＣ一定时间下ＮＯ产量（测代谢产物ＮＯ－２），采用Ｇｒｅｉｓ反应测定ＮＯ－２的量。结果１００ｎｍｏｌ／Ｌ１７β雌二醇作用１小时，ＮＯ－２即明显增高（Ｐ＜０．００１）；３０ｎｍｏｌ／Ｌ的１７β雌二醇作用下，４小时起ＮＯ－２产量即明显增高（Ｐ＜０．００１）；１０ｎｍｏｌ／Ｌ的１７β雌二醇作用１２小时及１ｎｍｏｌ／Ｌ的１７β雌二醇作用１８小时，ＮＯ－２产量均有明显增高（Ｐ＜０．０５）。０．１ｎｍｏｌ／Ｌ的１７β雌二醇作用２４小时内，ＮＯ－２产量与对照组比较无统计学差异（Ｐ＞０．０５）。１７β雌二醇作用的阈浓度可能在０．１ｎｍｏｌ／Ｌ－１ｎｍｏｌ／Ｌ之间。结论１７β雌二醇可促进原代人脐静脉内皮细胞ＮＯ的生成，可能为其抑制动脉粥样硬化形成、降低血管阻力的重要机理之一。

Objective To investigate the effects of estrogen on the production of nitric oxide (NO). Methods The primary cultures of human umbilical vein endothelial cells (HUVEC) were harvested by trypsin. Confluent primary cultures of HUVEC were exposed to 17 β estradiol in medium. The production of NO was assessed by Griess reaction. Results Exposure of the cells to 17 β estradiol (1nmol/L, 10nmol/L, 30nmol/L, 100nmol/L) enhanced the production of NO at 18 24 hours, 12 24 hours, 4 24 hours, 1 24 hours respectively ( P <0.01), however, the effect was not observed with 17 β estradiol at 0.1nmol/L within 24 hours.Conclusion 17 β estradiol could enhance the production of NO from the primary cultures of HUVEC. This finding suggests the antiatherosclerotic effect and the reduction of vessel resistance of estrogen.