摘要
采用脑薄片方法，观察损毁中缝背核（DR）、不同光照条件、5-羟色胺（5－HT）、氯氮平和利坦色林对视交叉上核（SCN）神经元昼夜自发放电的影响。结果：①SCN有昼高夜低的自发放电的节律性；②5－HT对SCN神经元自发放电有抑制作用，SCN神经元对5－HT的敏感性呈昼低夜高的节律（P＜0.01）；③氯氮平和利坦色林均能阻断5－HT对SCN神经元自发放电的抑制作用；④损毁DR后，SCN神经元对5－HT的敏感性增高，结果提示5－HT7样受体可能参与DR的5－HT能纤维对SCN神经元的调节作用。
The influence of serotonin(5 - HT)、 clozapine、ritanserin and dorsal raphe nucleus(DR) Iesion on spontaneous firing of SCN neurons under various illumination conditions was examined using a hypothalamic slice preparation. It was found that:①SCN neurons persist circadian rhythm in spontaneous discharge. The firing rate of SCN neurons is high during subjective day and low during subjective night.②5 - HT inhibits the spontaneous firing of SCN neurons and its sensitivity to 5 - HT in subjective day is lower than that in subjective night(p<0.01 ).③Both clozapine and ritanserin can block the effect of 5 - HT.④The sensitivity of SCN neurons to 5 - HT is higher after DR lesion. The results suggested that the inhibitary effect of 5 - HT proection form DR on SCN neuron discharge might be mediated by 5 - HT7, like receptor.
出处
《苏州医学院学报》
1998年第9期897-900,共4页
Acta Academiae Medicinae Suzhou
基金
国家自然科学基金!39470240
关键词
视交叉上核
5-羟色胺
神经元
昼夜节律
suprachiasmatic nucleus
dorsal raphe nucleus
5-hydroxytryptamine
clozapine
ritanserin
brain slice
