摘要
取健康儿童无菌外周血,用1.119g/ml淋巴细胞分离液分离细胞,制成单细胞悬液进行培养,培养开始时加入1,25双羟基维生素D3和碱性成纤维细胞生长因子,刺激促进成纤维前体细胞分化增殖,形成成纤维细胞集落,建立了培养方法。为儿童血循环中存在间充质干细胞的假说提供了初步实验依据,并为儿童骨骼、肌肉营养代谢性疾病和造血系统疾病研究提供了新的途径。
Human peripheral blood cells were obtained from two healthy childtgh donors. mhoheral blood monouclear cells and granulocytes were separated by centrifugation over a ficoll - hypaque density gradient(1. 119g/ml), suspended in the specific culture medium supplemented with 30% FCS, then plated in the flasks. With the addition of 1, 25 - dihydroxyvitaminD3 and basic fibroblast growth factor to enhance the differentiation of fibroblast - precursor and the proliferation of fibroblast, colony forming units - fibroblast (CFU - Fs) were formed. This result is the preliminary evidence for the hypothesis that the fibroblast - precursors are circulating in the peripheral blood of children. and this culture methed provides a new approach to the study of the nutritional and metabolic diseases of skeleton and muscles and haemopoietic diseases.
出处
《苏州医学院学报》
1998年第10期1015-1017,共3页
Acta Academiae Medicinae Suzhou
基金
院青年基金