稳消颗粒对兔实验性动脉粥样硬化单核细胞趋化蛋白-1及肿瘤坏死因子α表达的影响

Effect of Wenxiao Granule on the Expression of MCP-1 and TNF-α in Experimental Atherosclerosis Rabbits

目的观察稳消颗粒对兔实验性动脉粥样硬化单核细胞趋化蛋白-1 (MCP-1)和肿瘤坏死因子α (TNF- α )表达的影响,探讨其抗动脉粥样硬化的作用机制。方法将36只健康新西兰大白兔随机分为正常组、模型组、稳消颗粒组、辛伐他汀组。除正常组外,其余各组给与高脂饮食,建立兔动脉粥样硬化模型,根据体重比例折算临床剂量给予相应药物治疗。药物治疗8周后处死动物,取腹主动脉根部行免疫组化染色法,观察兔腹主动脉根部动脉粥样硬化MCP-1、 TNF- α的表达。结果与模型组比较,稳消颗粒组、辛伐他汀组MCP-1、 TNF- α的表达均明显下调(P<0.05);稳消颗粒组与辛伐他汀组间比较,差异无统计学意义(P> 0.05)。结论稳消颗粒可抑制MCP-1、 TNF- α的表达,益于动脉粥样硬化斑块防治与稳定。

Objective To observe the effect of Wenxiao Granule on the expression of MCP- 1 and TNF-α in experimental atherosclerosis rabbit, and explore its mechanism in treating atherosclerosis. Methods Thirty-six New Zealand rabbits were randomized to four groups： control group, Wenxiao granule group, Simvastatin group. They were fed a high-fat diet except normal group until the mature atherosclerotic plaques formed. The rabbits in two drug-treated groups were treated with respective drug for 8 weeks. All rabbits were sacrificed. The morphology and composition of atherosclerotic plaques in aortic roots were examined. The expression of MCP- 1 and TNF-α were determined by immunohistochemical staining. Results The expression of MCP-1 and TNF-α in plaque of Wenxiao Granule group and simvastatin group were significantly dereased compared with the model group （P〈0.05）, and there was no difference between the two drug-treated groups （P〉0.05）. Conclusion Wenxiao Granule can inhibit the expression of MCP-1 and TNF-α, which may stabilize plaque of atherosclerosis.