止咳平喘糖浆中盐酸麻黄碱的HPLC测定

Determination of Ephedrine Hydrochloride in Zhike Pingchuan Tangjiang by HPLC

目的采用HPLC法测定止咳平喘糖浆中盐酸麻黄碱的含量。方法采用外标一点法,Diamonsil ODS1 C_(18)柱,乙腈-0.1%磷酸溶液(含0.1%三乙胺) (3:97)为流动相,流速为1.0 mL/min,检测波长为205 nm。结果盐酸麻黄碱在012～0.96μg范围内呈良好线性关系,回归方程为Y= 109 759X+ 3 792.8,r=0.999 8,平均加样回收率为98.4%,RSD = 0.87% (n = 5)。结论本方法简便、准确,专属性强,测定结果重复性好,可用于止咳平喘糖浆中盐酸麻黄碱的定量分析。

Objective To establish the method for detemining the content of ephedrine hydrochloride in Zhike Pingchuan Tangjiang by HPLC. Methods Diamonsil ODS1 C18 Column was used with acetonitrile- 0.1% phosphoric solution （with 0.1% triehylamine） （3 ： 97） as the mobile phase, the detection wavelength as 205 nm, and flow rate was 1.0 mL/min. Results The calibration curve was linear at the range of 0.12 - 0.96 μg for ephedrine hydrochloride and linear equation was Y= 109 759X＋3 792.8, r=0.999 8. The average recovery was 98.4% and RSD was 0.87% （n =5）. Conclusion This method was simple, accurate and proper, with good reproducibility. It can be used for quantitative analysis of ephedrine hydrochloride in Zhike Pingchuan Tangjiang.