摘要
P16基因缺失在人类不同组织来源的细胞株和原发肿瘤中频繁发生,为探讨P16基因缺失与各型白血病的关系,采用PCR扩增技术检测了30例白血病,结果显示:17例ALL中有6例P16基因缺失,其中8例T-ALL中有5例、9例B-ALL中有1例P16基因缺失,而在5例CML、8例AML中P16基因存在。结果表明:P16基因缺失与ALL尤其是T-ALL有关。
Deletions of P16 gene frequently occurred in cell lines of different origin and human primary tumors. To figure out the association of P16 gene deletion with leukemia, we studied 30 cases of primary leukemias including 12 cases of ALL and 8 cases of acute myeloblastic leukemias(AML) and 5 cases of chronic myeloid leukemias(CML) by polymerase chain reaction(PCR), using Molt 4 cell line as positive control and HL 60 cell line as negative control. Our results indicate that six of the seventeen cases of ALL(35%) including five of the eight cases of T cell ALL(62.5%) and one of the nine cases of B cell ALL(11%) exhibited homozygous deletions of P16 gene but no deletion was found in any one of the 8 of AML, 5 of CML. These results demonstrate that the P16 gene homozygous deletion involved in ALL, especially in T ALL. Detection of P16 gene may be useful for the elucidation of pathogenesis of ALL.