摘要
目的建立空肠弯曲菌的快速检测方法。方法用PCR法扩增标本菌株中弯曲菌属16SrRNA片段、空肠弯曲菌M apA片段和结肠弯曲菌CeuE片段,通过电泳后对产物进行分析。结果11份标本中有7份检出弯曲菌属16SrRNA片段,其中3份样品中检测到空肠弯曲菌M apA片段,4份样品中检测到结肠弯曲菌CeuE片段。结论PCR技术可用于空肠弯曲菌的快速检测。
Objective To develop a rapid detection method for Campylobacter jejuni （C.jejuni）. Methods PCR method was used to amplify a group of specific 16SrRNA , MapA and CeuE fragments by electrophoresis after an analysis of the product. Results 7 of 11 specimens were detected Campylobacter 16SrRNA fragments, which were amplified containing 3 C. jejuni MapA fragments,4 Campylobacter coli（ C. coli） CeuE fragments. Conclusion The PCR technique can be applied to detect rapidly for C. jejuni and C. coli.
出处
《中国卫生工程学》
CAS
2009年第5期294-295,共2页
Chinese Journal of Public Health Engineering
关键词
PCR技术
空肠弯曲菌
检测方法
PCR technology
Campylobacter jejuni
Detection method
