HPLC法测定氯碘羟喹乳膏的含量

Determination of clioquinol in clioquinol cream by HPLC

目的：建立测定氯碘羟喹乳膏含量的HPLC法。方法：采用Zorbax C8柱（150mm×4．6mm，5μm）；流动相为乙二胺四醋酸二钠盐缓冲液（取乙二胺四醋酸二钠盐0．5g，加水400mL，振摇使溶解，加三乙胺4mL，用磷酸调节pH值至3．0）．甲醇（35：65），检测波长为254nm，流速1．0mL·min^-1。结果：氯碘羟喹进样量在0．06～1．20μg范围内呈良好的线性关系（r=0．9999），平均回收率为99．7％（n=9）。结论：与现行方法比较，该方法专属性强，适用于测定氯碘羟喹乳膏含量。

Objective：To establish a method for the determination of clioquinol in clioquinol cream by HPLC. Methods： The samples were separated on a Zorbax Cs column （150 mm× 4.6 mm, 5 μm）. The mobile phase was EDTA-2Na buffer solution （ dissolving 0.50 g of EDTA-2Na in 400 mL of water, adding 4.0 mL of triethylamine and mixing, using phosphoric acid to adjust pH value of 3.0） -methanol （35 ： 65 ） . The detection wavelength was at 254 nm, and the flow rate was 1.0 mL·min^ -1 Results ： Within the range of 0.06 - 1.20 μg, peak area and elioquinol injection quantity were in linear correlation （ r = 0. 999 9） and the average recovery were 99.7% （n =9）. Conclusion： Compared with the previous methods, the new method was more specific and can control quality of product effectively.