脂多糖增强芬太尼诱导离体兔肺动脉舒张反应

Lipopolysaccharide - pretreatment enhances fentanyl - induced relaxation in isolated rabbit pul- monary arteries

目的探讨阿片受体激动剂芬太尼对正常及内毒素休克时肺动脉血管反应性的影响，从而指导临床用药。方法应用血管环张力检测技术，观察芬太尼对正常及脂多糖（lipopo-lysacchafide，LPS）孵育的肺动脉血管环反应性的影响。结果在正常肺动脉组，芬太尼终浓度（0.1、1、10、100μmol／L）使去氧肾上腺素（Phenylephedfine，PE）预收缩的肺动脉舒张（EC50=1.699）；在LPS孵育的肺动脉组，芬太尼终浓度（1、10、100μmol／L）使PE预收缩的肺动脉舒张（EC50=2.496），芬太尼终浓度（0.1μmol／L）对肺动脉并没有影响；LPS组与正常组对照比较，LPS组芬太尼使肺动脉舒张反应Emax增加，在小剂量芬太尼终浓度（0.1、1μmol／L）时肺动脉的敏感度下降，在大剂量浓度（10、100μmol／L）时却使其敏感度增加。结论LPS可以增强芬太尼对肺动脉的最大舒张反应；并且LPS可使小剂量的芬太尼对肺动脉的敏感度下降，大剂量的芬太尼敏感度增加；LPS还可使芬太尼对肺动脉舒张反应的EC50增大。

Objective We investigated regulatory roles of fentanyl in isolated rabbit pulmonary arteries by lipopolysaccharide - preincubation. The goal was to assess the protective effect of ketamine on clinic septic shock patients. Methods Application of vascular ring tension detection technique to observe the effect of fentanyl on the normal and LPS（lipopolysaccharide, LPS） incubated pulmonary vascular. Results The pulmonary artery in the normal group, the final concentration of fentanyl （0.1μmol/L, 1μmol/L, 10μmol/L, 100μmol/L） to make pretreatment-PE pulmonary artery relaxation （EC50= 1.699） ; the pulmonary artery in the LPS group, the final concentration of fentanyl （1μmol/ L, 10μmol/L, 100μmol/L） to make pretreatment- PE pulmonary artery relaxation （EC50=2.496） , final concentration of fentanyl （ 0.1μmol/L） did not affect on pulmonary artery ; comparing LPS group with the normal control group , LPS group increased pulmonary artery Emax relaxation response, in the final concentration of low- dose fentanyl （0.1μmol/L, 1μmol/L） decreased the sensi- tivity of pulmonary artery, and in the high -dose concentration of 10μmol/L, 100μmol/L which increase the sensitivity. Conclusion LPS can enhance the effect of fentanyl on the maximal pulmonary artery relaxation reaction ; and small doses of LPS could decrease the sensitivity of the pulmonary artery to fentanyl , large doses of fentanyl increased the sensitivity ; LPS can also increase fentanyl on pulmonary artery EC50 response.