血小板相关抗体对巨核细胞增殖的实验研究

The Effect of Platelet-Associated Antibody on Megakaryocyte Proliferation

目的:探讨血小板相关抗体(platelet-associated antibody,PAIgG)对巨核细胞体外增殖的影响。方法:制备骨髓成纤维细胞无血清条件培养液,采用小鼠骨髓巨核细胞原代培养的方法,探讨PAIgG对巨核细胞体外增殖的影响。结果:50倍和100倍稀释的PAIgG能够显著抑制巨核细胞的增殖。结论:PAIgG对巨核细胞的体外增殖有抑制作用。

Objective：To investigate the effect of platelet-associated antibody （PAIgG） on the expansion of mature megakaryocytes in vitro. Methods：Serum-free bone marrow fibroblasts conditioned medium（F-CM） was prepared and collected. The method of bone marrow megakaryocytes primary culture was used and The effect of PAIgG on the expansion of megakaryocytes was discussed. Results： PAIgG that diluted for 50 times and 100 times can significantly inhibit megakaryocytes proliferation. Conclusion ： PAIgG can successfully inhibit megakaryocytes expansion in vitro.