环丙沙星对小鼠免疫毒性及肌肉品质影响

Effect of ciprofloxacin on immunotoxicity and muscle quality in rats

目的研究环丙沙星对小鼠免疫毒性和肌肉品质的影响。方法将小鼠分为对照组和环丙沙星高、中、低剂量组(44,11,5.5 mg/kg);每组10只动物,连续7 d腹腔注射给药,每日1次;通过计算小鼠胸腺和脾脏指数,观察脾淋巴细胞形态变化来探讨该药的免疫毒性;通过检测肌肉蛋白质含量、肌肉pH值,观察其对小鼠肌肉品质的影响。结果对照组胸腺和脾脏系数分别为(9.1±2.3),(6.3±1.1)mg/g,高剂量组胸腺和脾脏系数分别为(6.5±1.6),(3.4±0.5)mg/g,与对照组比较,差异均有统计学意义(P<0.05或P<0.01);而中、低剂量组的脾脏指数与对照组比较,差异均有统计学意义(P<0.05);环丙沙星各剂量组与对照组比较,肌肉pH值变化不明显;环丙沙星高、中、低剂量肌肉蛋白质含量分别为(24.68±0.55),(22.48±0.54),(21.22±1.25)g/100 g,与对照组(31.83±2.36)g/100 g比较明显下降(P<0.05);与对照组比较,高剂量组脾淋巴细胞形态异常。结论环丙沙星对小鼠具有一定的免疫毒性,明显减少了肌肉蛋白质含量。

Objective To study effect of ciprofloxacin on immunotoxicity and muscle quality in rats. Methods All rats in high, middle, low dose group （44,11,5.5 mg/kg） were administered with intraperitoneal injection of ciprofloxacin once a day,for 7 days continuously. The thymus and spleen coefficients were calculated and morphologic change of splenic lymphocyte was observed. The changes of muscle protein content and pH values were observed. Results Thymus and spleen coefficients （mg/g） in high and low dosage group were 9. 1 ± 2. 3,6. 3 ± 1.1, respectively, and those of in the controll were 6.5 ± 1.6,3.4 ± 0. 5. The contents of muscle protein （ g/100 g） in high, medium, and low dose groups were 24. 68 ±0.55,22.48 ± 0. 54,21.22 ±1.25, respectively, lower than that of the control （ 31.83 ＋ 2. 36, P 〈 0. 05 ）. Paramorphia of lymphocyte in high dose ciprofloxacin treated group was observed. Conclusion Ciprofloxacin has immunotoxicity and could reduce muscle protein significantly in rats.