污灌玉米有机提取物致小鼠肝肾氧化损伤作用

Organic pollutants-induced hepatic and renal oxidative damage in mice

目的研究某污灌区玉米中有机污染物对小鼠肝肾组织的氧化损伤作用。方法采用超声振荡法提取某污灌区农田生产的玉米中的有机污染物,对小鼠进行灌胃染毒2周;测定肝、肾组织的总超氧化物歧化酶(T-SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)。结果污灌区低、高剂量组肝组织的T-SOD活性分别为(32.44±7.93),(30.92±9.29)U/(mg.prot);肾组织的GSH-PX活性分别为(67.28±27.21),(66.52±15.19)U/(mg.prot),与对照组比较均明显降低(P<0.05或P<0.01)。结论该污灌区玉米有机提取物中含有致小鼠肝肾组织氧化损伤的有机污染物;对照区玉米中的有机污染物很可能来源于被污染的大气。

Objective To study hepatic and renal oxidative damage in mice exposed to organic extraction of maize from sewage irrigation area.Methods Organic extractions were obtained from maize collected in pollution area and contol area with supersonic method.Fifty mice were lavaged with the extractions once a day for 14 days and then the contents of malonaldehyde（MDA）,total superoxide dismutase（T-SOD）,glutathione-peroxide（GSH-Px） in liver and kidney tissues of the mice were determined.Results Compared with the reagent control group, T-SOD and GSH-Px in the liver weredecreased in the high-dose group （ P 〈 0. 01 ） ; T-SOD of the kidney were decreased. T-SOD, GHP-Px of the liver were decreased in the mice of high-dose group （ P 〈 0. 05 ）. Conclusion Organic extraction from maize from sewage irrigation area could induce hepatic and renal oxidative damage in mice.