肺癌组织中结核杆菌L-型感染检测

Detection of Mycobacterium tuberculosis L forms infection in tissues of lung carcinoma

目的检测肺癌组织中结核分枝杆菌L-型的感染状况,以分析结核分枝杆菌L-型在肺癌形成过程中的作用。方法选择肺癌患者手术切除后经石蜡包埋的组织标本共102份;采用原位分子杂交技术检测结核分枝杆菌L型MPB64基因片段的存在及定位;同时,用抗酸染色法检测标本中的结核分枝杆菌L-型。结果原位分子杂交检测石蜡包埋肺组织中MPB64基因片段,102份肺癌组织中结核菌阳性83例,阳性率为81.4%;染色检测结核L-型分枝杆菌,在102份肺癌组织中阳性69份,阳性率67.6%;原位分子杂交(81.4%)与染色法检出率(67.6%)比较,差异无统计学意义(P>0.05)。结论结核分枝杆菌L-型感染在肺癌的形成过程中可能起到一定作用。

Objective To determine the incidence,clinic epidemiological characteristics and the roles of Mycobacterium tuberculosis L forms infection in the diffusion and metastasis of lung carcinoma. Methods One hundred and two cases of formalin-fixed and paraffin-embeded tissue samples of surgically resected lung carcinomas were confirmed further by histopathological observation with hematoxylineosin（HE） staining. With MPB64 probe, the target gene and it＇s location in the tissue samples were detected with in situ hybridization technique. Meanwhile the Mycobacterium tuberculosis L forms in the sample was detected with intensified Kinyoun＇s acid fast staining. Results MPB64 gene fragment was detected in 83 of 102 lung cancinoma paraffin-embeded tissue（81.4% ）. Totally 67 Myobacterium taberculosis L forms positive cases were found in 102 lung cancinoma tissues （67.6%）. The results of the two methods had no significant dffference（P 〉0. 05）. Conclusion The incidence of Mycobacterium tuberculosis L forms infection in lung carcinoma suggests that the infection is likely to be involved in the occurrence or development of lung carcinoma.