高效液相色谱分离测定玉米类胡萝卜素

Separation and Determination of Carotenoids in Corn Grain by HPLC

应用高效液相色谱法分离测定玉米类胡萝卜素。结果表明：采用体积比为１５：７５：１０的己烷、丙酮、水在室温暗处浸提４８ｈ的制备样品方法，使用Ｎｏｖａ－ｐａｋＣ１８不锈钢柱，以体积比为８３：１５：２的乙腈、三氯甲烷、叔丁醇作流动相，流量线性梯度洗脱，柱温２５℃等色谱条件，在４４０ｎｍ波长下检测，使玉米中的主要类胡萝卜素在１０ｍｉｎ内得到了较好的分离。并以外标定量法对玉米中的４种主要类胡萝卜素进行了定量分析。

The carotenoids from corn grain were separated and determined by HPLC. Results showed that the main carotenoids in corn grain can be successfully separated in 10 min by employing Nova-pak C 18 stainless steel column, using acetonitrile-chloroform-tertiary butyl alcohol (volume ratio 83∶15∶2) as flow phase, applying linear gradient eluting, and using the column temperture of 25 ℃ and the detection wavelength of 440 nm after extracting the sample in hexane-acetone-water(volume ratio 15∶75∶10) for 48 h at room temperature in dark. Four kinds of main carotenoids from corn grain were determined by external standards.