脂联素基因多态性与缺血性脑卒中关系

Relationship between polymorphism of apml gene and ischemic stroke

目的探讨脂联素基因(apml gene)多态性与缺血性脑卒中的关系。方法采用病例对照组研究方法,利用荧光定量PCR的Taqman控针技术检测357例缺血性脑卒中新发病例及345例健康对照的脂联素基因(rs2241766、rs266729)多态性,用Logistic回归分析计算各基因型与缺血性脑卒中的相关性。结果rs2241766等位基因T→G突变增加了缺血性脑卒中发病无关(OR=1.55,P=0.005);rs266729多态性与总人群缺血性脑卒中发病无关(OR=1.13,P=0.57),但GG纯合突变增加了女性缺血性脑卒的发病风险(OR=3.25,P=0.04)。结论rs2241766基因多态性与我国东北汉族人群缺血性脑卒中的发病相关;rs266729等位基因GG纯合突变可能是我国东北汉族女性缺血性脑卒中的危险因素。

Objective To explore the association of apml gene polymorphisms and ischemic stroke. Methods A casecontrol study was conducted. Taqman probe technology of RT-PCR was applied to detect the genotype frequency of SNPs （rs266729 and rs2241766） of aprnl gene in 357 newly diagnosed ischemic stroke cases and 345 health controls. Logistic regression was used to evaluate the relationship between each genotype and ischemic stroke. Results The mutaion of rs2241766 （T→G） increased the risk of ischemic stroke （OR = 1.55, P = 0. 005 ）. The gene polymorphism of rs266729 was not related to the risk of ischemic stroke （OR = 1.13 ,P=0.57）. However,the genotype GG of rs266729 increased the risk of ischernic stroke in female population （ OR = 3. 25, P = 0. 04）. Conclusion The variance of rs2241766 in apml gene was related to the risk of ischemic stroke in Han nationality of northeast China and the genotype GG of rs266729 possibly increased the risk of ischemic stroke in female Han nationality of northeast China.