高效液相色谱法测定天麻含片中天麻苷含量

Determination of Gastrodin in Tianma Suckle Tablets by HPLC

目的：建立高效液相色谱法（HPLC）测定天麻含片中天麻苷（gastrodin）含量的方法，以期为天麻含片建立质量控制标准提供依据。方法：采用HPLC，以gastrodin为对照品，ZorbaxSB-C18键合硅胶柱（4．6mm×150mm，5μm），0．05％磷酸：乙睛（3：97）为流动相，流速1．0ml·min^-1，柱温为室温，检测波长220nm。结果：天麻苷在0．049～0．396ml^-1范围与峰面积呈良好线性关系，回归方程A=1．71×10^6＋5．864×10^7，r=0．9992（n=6）；测得2批天麻含片的天麻苷含量分别为1．0520mg／片和0．9692mg/片，平均回收率95．28％；方法精密度（RSD=0．98％，n=5）和重现性（RSD=0．78％，n=5）良好；天麻苷在4h内测定稳定RSD=1．16％。结论：该法用于测定天麻含片中天麻苷的含量，操作简便快速，结果准确可靠。

Objectives： To establish the HPLE menthod of determining content of gastrodin in Tianma Suckle Tablets in wanting to eontrol the quality.Methods： Gastrodin was taken as standard preparation.The analytical column was ZorbaxSB-C19 .The mobile phase was aeetonitrile： 0.05% phosphoric acid solution （97： 3） .The flow rate was 1.0ml·min^-1 and the detection wavelength was 22- 0nm.Results： the gastrodin has good linear relationship in the range ot0.066 - 0.396mg ·ml^-1 and the regression equation was A = 1.71 × 10^6 ＋ 5.864× 10^7C, r = 0.9992 （ n = 6}; the content of gastrodin in two different batch were 1.0520mg/tablet and 0.9692mg/ tablet. The average recovery was 95.28 % （ RSD = 1.22 % ）, the RSD of precision was 0.98 % and repeatability RSD was 0.78 %.The stability RSD was 1.16% and gastrodin was stable within 4h. Conclusion： The method is simple, accurate and reliable. It suitable for determination of gastrodin in Tianma Suckle Tablet.