亚抑菌浓度红霉素对临床分离表皮葡萄球菌黏附能力的影响

Effects of Subinhibitory Concentration of Erythromycin on Adhesion of Clinical Isolated Staphylococcus Epidermidis

目的:研究红霉素在亚抑菌浓度下对临床分离表皮葡萄球菌(SE)黏附能力的影响。方法:在药敏试验的基础上确定红霉素的亚抑菌药物浓度,以此浓度下的红霉素作用于代表菌株Se.015,采用微孔法测定光密度值以评价药物对菌株黏附能力的影响,电镜法观察样品中Se.015的黏附情况,同时设立空白溶剂为对照组。结果:在红霉素的亚抑菌浓度(4mg.L-1)下,与对照组比较,红霉素组光密度值升高(P<0.05),电镜下可见Se.015黏附量增加。结论:红霉素在亚抑菌浓度下可增强部分临床分离表皮葡萄球菌的黏附能力,提示其应用可能在特定条件下增加细菌生物膜感染的风险。

OBJECTIVE： To investigate the effect of subinhibitory concentration of erythromycin on adhesion of clinical isolated Staphylococcus（S .） epidermidis. METHODS： The subinhibitory concentration of erythromycin was determined based on the susceptibility test. and the representative strain Se.015 was treated with subinhibitory concentration of erythromycin. The optical density value determined by microtiter - plate assay was used to evaluate the effect of erythromycin on the adhesion of the representative strain Se.015, and electron microcopy was employed to observe the adhesion of Se.015 in samples with blank solvent served as control. RESULTS： Compared with control group, erythromycin（4 mg· L^-1） group showed significantly higher optical density value（P〈 0.05） and higher adhesion of Se.015 under electron microscope. CONCLUSION： Subinhibitory concentration of enrythromycin could increase the adhesion of some of clinical S. epidermidis stratus, suggesting the use of it may increase the risk of biofilm - associated infections under certain conditions.