化浊祛瘀汤治疗酒精性肝病的实验研究

Experimental Research of Huazhuo Quyu Tang on Treatmentof Alcoholic Liver Disease

目的探讨化浊祛瘀汤对酒精性肝病大鼠影响的机理。方法通过乙醇梯度灌胃的方法建立酒精性肝病(ALD)大鼠模型,并使用化浊祛瘀汤给大鼠灌胃,观察化浊祛瘀汤对酒精性肝病肝损伤大鼠NF-κB表达,NF-α、CAT等水平的影响,并进行HE染色,观察肝脏组织基本病理改变。结果模型组大鼠NF-κB表达明显增强(P<0.01),TNF-α含量显著增加(P<0.01),CAT含量明显降低(P<0.01);化浊祛瘀汤治疗组NF-κB表达明显减弱,TNF-α含量明显降低(P<0.01),CAT含量明显升高(P<0.01);中药高剂量组与西药组比较有显著性差异(P<0.01)。结论化浊祛瘀方药能够抑制酒精性肝病时NF-κB、TNF-α的表达,改善ALD脂质过氧化过程。

Objective To explore the mechanism of Huazhuo Quyu Tang on alcoholic liver disease （ALD） in rats. Methods Alcohol - gradient gastric perfusion was used to establish ALD rat model. Huazhuo Quyu Tang was administered by gastric perfusion in rats to observe the impacts of the decoction on NF -κB expression and the levels of TNF - α and CAT in ALD rats. By HE staining, the basic pathological changes of liver tissue was observed. Results In model group, NF -κB expression increased apparently （ P 〈0.01） , TNF -α level increased apparently （P 〈 0.01 ） and CAT reduced apparently （P 〈 0.01）. In Huazhuo Quyu Tang treatment group, NF - κB expression decreased apparently（ P 〈0.01） , TNF - α level reduced apparently（ P 〈 0.01 ）and CAT increased apparently（ P 〈 0.01 ）. The significant difference presented in comparison between high-dose Chinese herb group and western drug group （P 〈 0.01 ）. Conclusions Huazhuo Quyu Tang can inhibit the expressions of NF - κB and TNF -α and improve lipid peroxidation of ALD.