不同病期胃癌组织与癌旁正常胃黏膜中TSPYL5 mRNA表达的比较

Comparison of the expression of TSPYL5 mRNA in gastric carcinoma and normal tissues at different clinic stages

目的:探讨胃癌组织及其邻近胃组织中睾丸特异性Y样蛋白5(testis-specific Y-like protein5,TSPYL5)的表达对胃癌生物学行为的影响。方法:采用RT-PCR方法检测46例胃癌组织、癌旁胃正常粘膜组织中TSPYL5 mRNA的表达,分析其表达与胃癌生成、淋巴结转移、肿瘤浸润程度及分级的关系,同时采用MTT方法检测TSPYL5对胃癌细胞株AGS的影响。结果:与癌旁组织相比,46例胃癌组织中有26例TSPYL5 mRNA表达显著下调(56.5%),有淋巴结转移的胃癌组织中TSPYL5表达率与无淋巴结转移组织相比无显著差异(P>0.05),而在TNM中的Ⅱ、Ⅲ期以及不存在腹腔和远处转移时TSPYL5 mRNA表达显著下调(P<0.05)。同时TSPYL5对AGS细胞株生长有抑制作用。结论:TSPYL5 mRNA表达下调可能与胃癌的发生发展相关。

Objective：To study the expression of TSPYL5 in gastric carcinoma and adjacent gastric tissues,and the effect of the expression of TSPYL5 on the biobehavion of gastric carcinoma. Methods：RT-PCR was used to detect the expression of TSPYL5 mRNA in 46 samples of human gastric cancer and normal gastric tissue, and the relationship between the expression of TSPYL5 and oncogenesis, lymph node metastasis, depth of tumor invasion and grade in gastric carcinoma was also analyzed. Meanwhile the effeet of TSPYL5 on AGS cells was analyzed by MTT in vivo. Results：Of 46 gastric cancer tumor tissues,26 tissues were detected the expression of TSPYL5 mRNA. The rate of TSPYL5 mRNA in the primary gastric cancer tissues was significantly higher than that in the adjacent non-cancerous tissues （56.5%）. The TSPYL5 positive isolates had more lymph node metastases than that of TSPYL5 negative ones （P 〉 0.05）. Furthermore, the expression of TSPYL5 mRNA in TNM and none metastasis signifcantly decreased （P 〈 0.05）. TSPYL5 suppressed the growth of AGS cells. Conclusion：TSPYL5 may be had a significance effect on development and progress in gastric cancer.