N-乙酰半胱氨酸对糖尿病肾病大鼠炎症反应的抑制作用

N-acetylcysteine inhibits the inflammation in diabetic nephropathy rats

目的:探讨N-乙酰半胱氨酸(NAC)对糖尿病肾病(DN)大鼠炎症反应的抑制作用。方法:制备DN大鼠模型,将动物随机分为正常对照组、DN组和NAC组。8周后测定尿白蛋白排泄率,血清高敏C反应蛋白(hs-CRP)、白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)水平,免疫组化方法检测肾组织NF-κB蛋白表达水平,观察肾脏病理标本。结果:①与正常对照组比较,DN组和NAC组尿白蛋白排泄率增多(P<0.01),血清hs-CRP、IL-6和TNF-α水平升高(P<0.01或P<0.05),肾组织NF-κB蛋白表达水平升高(P<0.01或P<0.05),肾脏病理改变明显;②与DN组比较,NAC组尿白蛋白排泄率减少(P<0.01),血清hs-CRP、IL-6和TNF-α水平降低(P<0.05),肾组织NF-κB蛋白表达水平降低(P<0.05)和肾脏病理改变较轻。结论:NAC对DN大鼠肾脏有保护作用,其部分机制与抑制炎症反应有关。

Objective：To investigate the inhibition of N-acetylcysteine （NAC） on the inflammation of diabetic nephropathy rats. Methods：A rat model of DN was established. The rats were divided into control group, DN group and NAC group randomly. Urinary albumin excretion rate （UAER）,hs-CRP,IL-6 and TNF-α in serum were determined. The expression of NF-κB in renal tissue was detected by immunohistochemistry 8 weeks after treatment. Pathomorphologic changes of nephridial tissues were observed. Results：① Compared to control group, UAER, hs-CRP, IL-6 and TNF-α in serum were significantly higher（P 〈 0.01 or P 〈 0.05）, activity of NF-κB in nephridial tissue increased （P 〈 0.01 or P 〈 0.05） and significant changes of pathomorphology in nephridial tissues were observed in DN group and NAC group. ②Compared to DN group, UAER,hs-CRP, IL-6 and TNF-α in serum were significantly lower（P 〈 0.05）, activity of NF-κB in nephridial tissue was lower （P 〈 0.05） and improvement of pathomorphology in nephridial tissues were observed in NAC group. Conclusion：The protective effect of NAC on the kidney of the DN rats may be partly related with the inhibition on inflammation.