川芎嗪对新生鼠缺血缺氧脑损伤HIF-1α表达的影响

The effect of liguistrazine on the HIF-1ɑ expression in neonatal rats with hypoxic ischemic brain damage

目的:探讨实验性大鼠缺血缺氧脑损伤(HIBD)时低氧诱导因子-1α(HIF-1α)的表达及川芎嗪对HIF-1ɑ表达的影响。方法:新生SD大鼠结扎左侧颈总动脉并置于缺氧环境中,制备HIBD模型。川芎嗪干预组在缺血前和缺血后腹腔注射川芎嗪(50mg/kg)。应用逆转录PCR和免疫组化方法观察川芎嗪对HIBD新生鼠皮质部位HIF-1α mRNA及蛋白表达的影响。结果:缺血缺氧2h后HIF-1α mRNA及蛋白表达有增加的趋势,但与假手术组相比无统计学意义(P>0.05);川芎嗪干预组HIF-1α mRNA及蛋白表达显著增加,明显高于缺血缺氧组(P<0.05)。结论:川芎嗪促进新生鼠缺血缺氧脑损伤皮质部位HIF-1α mRNA及蛋白表达,是其神经保护作用的重要机制。

Objective：To explore the effect of liguistrazine on the expression of hypoxia-induced factor-1α（HIF-1α）in neonatal rats with hypoxic-ischemic brain damage （HIBD）. Methods：Models of 7-day-old neonatal rats with HIBD were established by occluding the left common carotid artery and subjected to inhalation of 8% oxygen. The mice of treated group were injected with liguistrazine intraperitoneally （50 mg/kg） before and after the operation. The expression of HIF-1α in cortex was measured by RT-PCR and immunohistoehemical staining. Results： Although the levels of HIF-1α mRNA and protein expression at 2 h after HIBD were higher than those of sham group,there was no significance（P 〉 0.05） .While the expression of HIF-1α mRNA and protein were remarkably higher in liguistrazine treated group than those of HIBD group （P 〈 0.05）. Conclusion：Liguistrazine can increase the expression of HIF-1α mRNA and protein in neonatal rats with HIBD,which may be the molecular mechanism of its neuroprotective effect.