KMnO_4-甲醛流动注射化学发光法测定木犀草素

Determination of luteolin by flow injection chemiluminescence with potassium permanganate-formaldehyde system

在酸性介质中,木犀草素在KMnO4-HCHO体系中产生化学发光,据此建立了一种简便、快速测定木犀草素的化学发光新方法。化学发光强度与木犀草素的浓度在5.0×10^-8-1.5×10^-5g/mL范围内呈现出良好的线性关系。其检测限（3σ）为3.0×10^-8g/mL,对8.0×10^-7g/mL的木犀草素溶液进行11次平行测定,相对标准偏差为2.8%。方法可应用于实际样品和合成样品中木犀草素的测定。并探讨了反应的机理。

A flow injection chemiluminescence method for the determination of luteolin, which is based on the enhancement effect of the chemiluminescence produced from the acidic potassium permanganate-formaldehyde system, is described in this paper. Under optimized conditions, the relationship between the enhanced CL intensity and the concentration of luteolin is linear in the ranges of5.0×10^-8-1.5×10^-5g/mL and 3.0×10^-6 - 1.5×10^-5 g/mL with a detection limit （3σ） of 3.0 ×10^-8 g/mL. Relative standard deviation （RSD） is 2.8% （n = 11） for 8.0×10^-7 g/mE luteolin. The proposed method has been successfully applied to the determination of luteolin in real and synthetic samples with satisfactory results. The mechanism of the reaction is also discussed.