UPLC法测定浓缩苹果汁中阿维菌素残留量

Determination of avermectin residue in concentrated apple juice by UPLC

建立了超高效液相色谱法（UPLC）测定浓缩苹果汁阿维菌素残留的方法。样品经C18固相萃取柱（250×4.6μmi.d.,5 mm）净化分离,以V（乙腈）∶V（水）=80∶20为流动相,245 nm波长检测,流速0.3 mL/min。结果表明：本法测定的阿维菌素在4 min内可以完全分离,线性范围为0.01-200 mg/L,R2=0.9997,当添加量为0.05、0.5、1.0 mg/kg时,回收率为99.5%-108.1%,RSD为0.30%-1.1%,测定下限为0.005 mg/kg。方法可用于浓缩苹果阿维菌素残留量测定。

The ultra-performance liquid chromatography with a photodiode army detector （UPLC-PDA） was used for the determination of avermectin in concentrated apple juice. Good linearity of avermectin was obtained over the ranges of 0.01 - 200 mg/L （RE = 0.9997）. The results suggested that the recoveries of avernection from samples spiked with 0.05, 0.5, 1.0 mg/kg standards ranged from 99.50% to 108.1%, with the coefficients of variation of 0.30% to 1.1%, and the limit of detection was 0. 005 mg/kg. This method was simple quick and sensitive, and could meet the requirements for the detection of the avermectin residues in concentrated apple juice.