摘要
建立了超高效液相色谱法(UPLC)测定浓缩苹果汁阿维菌素残留的方法。样品经C18固相萃取柱(250×4.6μmi.d.,5 mm)净化分离,以V(乙腈)∶V(水)=80∶20为流动相,245 nm波长检测,流速0.3 mL/min。结果表明:本法测定的阿维菌素在4 min内可以完全分离,线性范围为0.01-200 mg/L,R2=0.9997,当添加量为0.05、0.5、1.0 mg/kg时,回收率为99.5%-108.1%,RSD为0.30%-1.1%,测定下限为0.005 mg/kg。方法可用于浓缩苹果阿维菌素残留量测定。
The ultra-performance liquid chromatography with a photodiode army detector (UPLC-PDA) was used for the determination of avermectin in concentrated apple juice. Good linearity of avermectin was obtained over the ranges of 0.01 - 200 mg/L (RE = 0.9997). The results suggested that the recoveries of avernection from samples spiked with 0.05, 0.5, 1.0 mg/kg standards ranged from 99.50% to 108.1%, with the coefficients of variation of 0.30% to 1.1%, and the limit of detection was 0. 005 mg/kg. This method was simple quick and sensitive, and could meet the requirements for the detection of the avermectin residues in concentrated apple juice.
出处
《分析试验室》
CAS
CSCD
北大核心
2009年第11期74-76,共3页
Chinese Journal of Analysis Laboratory