高效液相色谱分析七种氟苯甲酸示踪剂

Study on the detection of fluoric-benzoic acids tracers by high performance liquid chromatography

建立了高效液相色谱法测定7种新型水文示踪剂氟苯甲酸的分析方法。使用C18（150 mm,4.6 mmi.d,5μm）色谱柱、乙腈∶水（磷酸盐）=20∶80（2.56 mmol/L）为流动相、紫外检测器,检测波长223 nm。考察了pH、缓冲溶液浓度、有机相浓度等对保留时间的影响。相对标准偏差1.2%-4.2%,线性范围0.05-5.6μg/L。方法的检出限为0.03-0.07μg/L。平均回收率93.3%-106.6%之间。

A method for the determination of seven fluoric-benzoic acid tracers was established by high performance liquid chromatography （HPLC）. The seven fluoric-benzoic acid tracers were separated on a C18 （150 mm, 4.6 mm i. d, 5μm） column with acetonitrile-water （sodium phosphate） = 20：80 （2.56 mmol/L） as the mobile phase, and UV detection at 223 nm. The effects of pH values, the concentrations of organic phase, and the concentrations of buffer on separation were investigated. Linear ranges were from 0.05 to 5.6 μg/L. The detection limits were 0.03 - 0.07 μg/L. The recoveries were between 93.3% and 106.6%, and the relative standard deviations （RSD） were from 1.2% to 4.2%.