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海带有性繁殖遗传机制的研究(英文) 被引量:1

Genetic mechanism on sexual reproduction of Laminaria
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摘要 以日本长海带(Laminaria longissima)×真海带(Laminaria japonica)杂交F1代60株幼孢子体为材料,利用RAPD、ISSR和AFLP 3种分子标记技术进行海带分子遗传机制的研究。结果表明:由25个RAPD引物、14个ISSR引物和20对AFLP引物组合共扩增出151个RAPD位点、89个ISSR位点和532个AFLP位点。其中符合尔孟德遗传分离规律的RAPD、ISSR和AFLP位点分别有125、87和430个,各占每种标记总扩增位点数的83.3%、97.8%和80.8%;偏离孟德尔遗传分离规律的RAPD、ISSR和AFLP位点分别有25、2和102个,各占每种标记总扩增位点数的16.7%、2.2%和19.2%。上述统计结果从分子水平上验证了海带有性繁殖过程符合孟德尔遗传方式。 To investigate the genetic mechanism during the Laminaria sexual reproduction, the gametophytes of L. japonica Aresch and L. longissima Miyabe were chosen as parents for hybridization. RAPD, ISSR and AFLP makers were used for the analysis of genetic mechanism. All the 151 RAPD loci were amplified with 25 random primers ,89 ISSR loci were amplified with 14 SSR primers ,and 532 AFLP loci were amplified with 20 selective primer combinations. Among the amplified loci, 125 RAPD loci, 87 ISSR loci and 430 AFLP loci were fit for Mendel' s laws of inheritance and segregation among the F1 progenies, which accounted for 83.3%, 97.8% and 80.8%, respectively. It showed that the sexual reproduction of Laminaria basically followed Mendel's laws of inheritance and segregation.
出处 《安徽农业大学学报》 CAS CSCD 北大核心 2009年第4期552-556,共5页 Journal of Anhui Agricultural University
基金 国家自然基金项目(40376049,30500383) 合肥学院引进人才基金项目(RC024)共同资助
关键词 海带 遗传机制 AFLP标记 ISSR标记 RAPD标记 Laminaria genetic mechanism AFLP ISSR RAPD
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  • 1R.Moav,祝培福,余先觉.水产养殖业中的遗传改良[J].淡水渔业,1977,7(9):21-34. 被引量:2
  • 2Ammiraju JSS, Dholakia BB, Santra DK et al. (2001). Identification of inter simple sequence repeat (ISSR) markers associated with seed size in wheat. Theor Appl Genet 102,726-732.
  • 3Arnedo-Andres MS, Gil-Ortega R, Luis-Arteaga M, Hormaza JI (2002). Development of RAPD and SCAR markers linked tothe Pvr4 locus for resistance to PVY in pepper (Capsicum annuum L.). Theor Appl Genet 105,1067-1074.
  • 4Billot C, Rousvoal S, Estoup A et al. (1998). Isolation and characterization of microsatellite markers in the nuclear genome of the brown alga Laminaria digitata (Phaeophyceae). Mol Ecol 7,1778-1790.
  • 5Casasoli M, Mattioni C, Cherubini M, Villani F (2001). A genetic linkage map of European chestnut (Castanea sativa Mill.) based on RAPD, ISSR and isozyme markers. Theor Appl Genet 102,1190-1199.
  • 6Fang DQ, Roose ML (1997). Identification of closely related citrus cultivars with inter-simple sequence repeat markersTheor Appl Genet 95,408-417.
  • 7Fang ZX, Dai JX (1980). The use of hapiloid phases in the genetic study of Laminariajaponica. Acta Genet Sin 7,19-25 (in Chinese with an English abstract).
  • 8Felsenstein J (1989). PHYLIP: Phylogeny inference package.Cladistics 5,164-166.
  • 9Gilbert JE, Lewis RV, Wilkinson M J, Caligari PDS (1999). Developing an appreciate strategy to assess genetic variability in plant germplasm collections. TheorAppl Genet 98,1125-1131.
  • 10Gosselin I, Zhou Y, Bousquet J, Isabel N (2002). Megagametophyte-derived linkage maps of white spruce (Picea glauca) based on RAPD, SCAR and ESTP markers. TheorAppl Genet 104,987-997.

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