家蚕UDP葡萄糖基转移酶Bmugt2基因的克隆与RNA干涉(英文)

Cloning and RNAi of Bmugt2,an UDP-glucosyltransferase gene in Bombyx mori

糖基化在大多体内复合物的失活和排出过程中起重要的作用。报道家蚕一UDP葡萄糖基转移酶基因Bmugt2的特征和可能的功能。该基因编码序列长1 578 bp(登录号:FJ237534),翻译525个氨基酸,推定的氨基酸与UGT家族的其它氨基酸有约30%的一致性。RT-PCR检测该基因主要在家蚕丝腺中表达,应用体外转录合成的基因dsRNA干涉家蚕后,丝腺中基因的表达明显降低,但对家蚕茧色无明显的可见影响。

Glucosidation plays a major role in inactivation and excretion of most compounds. We report here the characterization and RNAi of an UDP-glucosyhransferase gene in silkworm, namely Bmugt2. The coding sequence is 1 578 bp in length （ GenBank accession number ： FJ237534 ） and can be translated into a protein of 525 amino acids. The deduced protein has about 30% overall amino-acid identity with other members in UGT family. RT-PCR result shows that the gene has high expression in silk gland in all the tissues tested and the expression was suppressed distinctly by corresponding dsRNA through microinjection into silkworm. However, the RNAi of the gene has no obvious effect on the cocoon color.