白介素-6和吡格列酮对脂肪因子URB表达的调控

STUDY OF REGULATING EFFECTS OF INTERLEUKIN-6 AND PIOGLITAZONE ON URB GENE EXPRESSION

目的探讨3T3-L1前脂肪细胞分化过程中新基因URB[(upregulated in bombesin receptor subtype-3(BRS-3)-deficient mice,缺乏铃蟾肽受体亚型3小鼠上调因子)的表达情况,以及在不同时点白介素-6(Interleukin-6,IL-6),吡格列酮(Pioglitazone,PIO)对其调节的作用。方法以不同浓度IL-6和PIO于各时段处理3T3-L1细胞,利用实时定量RT-PCR技术检测各条件下URB mRNA水平。同时油红O染色鉴定脂滴形成。结果(1)在正常分化过程中,URB mRNA和脂联素mRNA水平都是逐渐升高的。(2)IL-6对分化过程及分化成熟后脂肪细胞中URB基因的表达均具抑制作用,且呈剂量依赖性。(3)PIO能促进成熟后脂肪细胞的URB的表达,并改善IL-6的抑制作用。结论URB可能是影响能量代谢的一个重要脂肪因子和潜在的药理学靶位。

Objective To observe the expression of novel URB gene in the differentiation display of 3T3-L1 preadipocytes and the effects of interleukin-6 （IL-6） and pioglitazone （PIO） on URB mRNA expression in 3T3-LI adipocytes at different times. Method The 3T3-L1 adipocytes were treated with IL-6 and PIO under different concentration and different time phase, and real-time fluorescence monitoring RT-PCR was conducted to measure the URB mRNA level. Fat droplets were verified by oil red O staining. Results 1. URB mRNA level was increased gradually in the whole differentiation process. 2. The expression levels of URB mRNA was decreased along with the treatment of IL-6 in a dose-dependent manner in 3T3-L1 preadipocytes and adipocytes. 3. PIO increased the level of URB mRNA in 3T3-LI adipocytes, and the reducing effect of IL-6 on URB mRNA could be corrected by PIO. Conclusion URB may be an important adipocytokin which affects energy metabolism and may work as a pharmacological gene target in the future.