紫茎泽兰hsp90和hsp17.66基因启动子的克隆及其序列分析

Cloning and Sequence Analysis of Promoters in hsp90 and hsp17.66 Genes from Ageratina adenophora

以入侵植物紫茎泽兰(Ageratina adenophora Sprengel)为材料,采用基于PCR方法的染色体步行和改良的热不对称交错PCR(thermal asymmetric interlaced PCR,TAIL-PCR)两种方法分别克隆到hsp90和hsp17.66基因的5′上游启动子序列,长度分别为864 bp和1 485 bp(GenBank登录号分别为FJ434253和FJ434252)。测序结果表明:这两个启动子序列均具有hsp启动子特有的HSE元件,及其他一些启动子顺式作用元件,如TATA-box,CAAT-box等。

The promoter region of hsp90 with 864 bp （GenBank No. FJ434253）, from invasive weed Ageratina adenophora was amplified based on PCR methods, which are available as chromosome walking from a known sequence to an unknown region. Moreover, another promoter region of hsp17.66 with 1 485 bp （GenBank No. FJ434252） was cloned by thermal asymmetric interlaced PCR （TAIL-PCR）. Both of the 5＇-flanking regions of hsp90 and hsp17.66 contained specific HSE element and several cis-acting elements, such as TATA-box, CAAT-box,et al.