RECK在成釉细胞瘤中的表达及其与基质金属蛋白酶-2的关系

Expression of RECK in ameloblastoma and its relationship with matrix metalloproteinase-2

目的:研究RECK(reversion-inducing cysteine rich protein with Kazal motifs)和基质金属蛋白酶-2(matrixmetalloproteinase-2,MMP-2)在成釉细胞瘤(ameloblastoma,AB)中的表达及其相关性,探讨两者与成釉细胞瘤临床生物学行为的关系。方法:应用免疫组化EliVisionTM plus法,检测69例成釉细胞瘤(原发AB45例,复发AB24例)、6例成釉细胞癌和16例牙源性角化囊性瘤(keratocystic odontogenic tumor,KCOT)中RECK、MMP-2蛋白的表达,采用SPSS13.0软件包对数据进行统计学分析。结果:RECK在KCOT、AB和成釉细胞癌中的阳性表达率依次降低,组间比较差异均有统计学意义(P<0.05),且复发AB的RECK表达显著低于原发AB(P<0.01)。MMP-2在AB和成釉细胞癌中的阳性表达率均显著高于KCOT(P<0.05),且MMP-2的表达在AB与成釉细胞癌以及原发AB与复发AB间均无显著性差异(P>0.05)。RECK与MMP-2在AB中的表达呈负相关(r=-0.431,P<0.001)。结论:RECK与AB的临床生物学行为密切相关,可能通过调控MMP-2参与AB的浸润、复发和恶性转化过程。

PURPOSE：To investigate the expression of RECK and matrix metalloproteinase-2 （MMP-2） in ameloblastoma （AB） and the relationship between them, and explore their role in the clinical biologic behavior of AB. METHODS： EliVisionTM plus 2-step immunochemistry staining was employed to detect protein expression of RECK and MMP-2 in the paraffin-embedded specimens of 69 ABs （including 45 primary ABs and 24 recurrent ABs）, 6 ameloblastic carcinomas and 16 keratoeystie odontogenic tumors （KCOT）.The data was analyzed with SPSS13.0 software package. RESULTS： The expression of RECK in AB decreased compared with KCOT （P〈0.05）, and there was nearly no expression in ameloblastic carcinomas.The RECK expression showed down-regulated in recurrent AB compared with primary AB （P〈0.01）. The expression of MMP-2 in AB and ameloblastic carcinoma increased compared with KCOT （P〈0.05）, and there was no significant difference between primary AB and recurrent AB, and the same with AB and ameloblastic carcinoma （P〉0.05）. RECK protein expression had a negative relationship with MMP-2 expression in AB （r =-0.431,P〈0.001）. CONCLUSION： RECK may play an important role in the clinico-biological behavior of AB, and it may participate the invasion, recurrence and malignant transformation of AB by regulating MMP-2.