摘要
建立蒙古绵羊输卵管上皮细胞培养体系作为体外实验模型,分别添加10-6、10-7、10-8、10-9和10-10mol/L孕酮,运用实时荧光定量PCR方法测定孕酮对上皮细胞内β-防御素相对表达量的影响。结果显示,与对照组比较,10-6和10-7mol/L孕酮组β-防御素相对表达量显著升高(P<0.05);10-8和10-9mol/L孕酮组极显著升高(P<0.01);而10-10mol/L孕酮组未见显著性差异。孕酮添加组间比较显示,10-8和10-9mol/L孕酮组极显著高于10-10mol/L组(P<0.01),其它孕酮添加组间未见显著性差异。分析认为,一定浓度的孕酮(10-9-10-6mol/L)对培养的输卵管上皮细胞β-防御素的表达有促进作用。且不同浓度的孕酮对β-防御素表达的影响程度不同。因而推断,雌性生理周期下,雌性生殖道β-防御素的表达与孕激素相关。
The epithelial cell of the Mongolian sheep＇s fallopian tubes were cultured as in vitro model, different concentrations of progesterone （10^-6, 10^-7, 10^-8, 10^-9 and 10^-10mol/L） were added in the culture, and then the relative expressions of sheep β-defensin-1 （sBD-1） mRNA were surveyed by real time PCR. The results showed that, compared with the control group, the relative expressions of sBD-1 mRNA added with 10^-6 and 10^-7mol/L progesterone were significantly higher （P〈0.05）, and those of 10^-8 and 10^-9mol/L groups were remarkably higher （P〈0.0 1）. However, there was no significant difference between 10^-10mol/L group and control. Among groups added with progesterone, the sBD-1 mRNA expressions of 10^-8 and 10^-9mol/L groups were markedly higher than that of 10^-10mol/L group （P〈0.01）, but there was no difference among other added groups. The results indicated that progesterone promoted the relative expression of sBD-1 mRNA in the epithelial cell of the fallopian tubes within the indicated concentrations （10^-9-10^-6 mol/L）. And the relative expression of sBD-1 mRNA was varied with the different concentrations of progesterone. In conclusion, the expression of β-defensin was related to progesterone in female reproductive tract.
基金
国家自然科学基金资助项目(30460093)
