血管内皮生长因子反义核酸增强华蟾素诱导K562细胞凋亡

VEGF antisense oligodeoxynucleotides in enhancing the apoptosis-inducing effect of Cinobufotalin on K562 cells

目的研究VEGF反义核酸(ASON)增强华蟾素对人慢性粒细胞白血病K562细胞株诱导凋亡效应。方法合成VEGF ASON转染入K562细胞,4种浓度的华蟾素(1、3、5、7 mg/L)作用于K562细胞株24、48、72 h,应用West-ern blot法检测VEGF蛋白的表达,原位细胞凋亡(TUNEL)、流式细胞仪法(FCM法)检测细胞凋亡。结果不同剂量华蟾素组对K562细胞株均有抑制作用,并具有时间和浓度依赖性。经VEGF ASON转染的K562细胞株对华蟾素的诱导凋亡作用更加明显。结论华蟾素在体外一定浓度范围内可诱导K562细胞株凋亡,VEGF ASON可增强华蟾素诱导K562细胞凋亡的作用。

Objective To study the vascular endothelium growth factor （VEGF） antisense oligonucleotide （ASON） Cinobufotalin in enhancing apoptosis induced by human chronic myeloid leukemia K562 cell line. Methods The synthesis of VEGF ASON was transfected into the K562 cells; Cinobufotalin of four concentrations （1, 3, 5 and 7 mg/L） acted on the K562 cell line for 24 h, 48 h and 72 h. Western blot was used to detect VEGF protein expression, while in situ apoptosis （TUNEL） and flow cytometry method （FCM method） were employed to detect the apoptosis. Results The different doses of Cinobufotalin all inhibited K562 cell line in time- and dose- dependent manners. K562 cell line transfected by VEGF ASON had a more pronounced induction of apoptosis. Oonclusion The in vitro Cinobufotalin at a certain range of concentration can induce apoptosis in K562 cell line, and VEGF ASON enhances Cinobufotalin＇s effect in inducing apoptosis of K562 cells.