人结肠癌中AKT2和PTEN蛋白表达的意义及相关性

Significance of AKT2 and PTEN protein expressions and their correlation with human colon cancer

目的检测AKT2蛋白在人结肠癌组织中的表达及其与PTEN的相关性。方法应用免疫组织化学SP法检测30例正常结肠、30例腺瘤和64例结肠癌组织中AKT2和PTEN的表达。结果从正常组织、腺瘤组织到结肠癌组织,PTEN的阳性表达率呈递减趋势,AKT2呈递增趋势;PTEN在正常结肠组织中的表达明显高于结肠腺瘤和结肠癌组织中的表达,差异有统计学意义(χ2=68.855,P<0.05),而AKT2在结肠腺瘤和结肠癌组织中的表达明显高于正常结肠组织中的表达,差异有统计学意义(χ2=37.574,P<0.05);随结肠癌分化程度降低、淋巴结的转移、Dukes分期的增加,AKT2的阳性表达率升高(P<0.05);PTEN与AKT2的表达呈负相关(r=-0.143,n=64,P<0.05)。结论由于PTEN表达下调或缺失而导致AKT2的异常高表达,AKT2的高表达与结肠癌的发生发展有关。

Objective To detect the expression of AKT2 in human colon cancer and understand its relationship with PTEN. Methods The expressions of AKT2 and PTEN were detected in 30 patients with normal colonic tissues, 30 patients with colon adenoma tissues, and 64 patients with colon carcinoma tissues by immunohistochemical SP staining method. Results The positive expressive rate of PTEN presented a trend of progressive decrease from normal tissues, adenoma tissues to colon carcinoma tissues, while the positive expression rate of AKT2 presented a trend of progressive increase. PTEN expression was obviously higher in normal colon tissues than in colon adenoma tissues and colon cancer tissues （ x2 =68. 855, P〈O. 05）. However, the expression of AKT2 in colon adenoma tissues and colon cancer tissues was significantly higher than that in normal colon tissues （ x2 =37. 574, P〈0.01）. With the decrease of differentiation degree in colon cancer, metastasis of lymph node and increase of Dukes staging, the positive expression rate of AKT2 increased （P〈O. 05）. AKT2 and PTEN expressions were negatively correlated （ r = 0. 143, n = 64, P 〈 0.05）. Conclusion The results demonstrate that down- regulation or loss of PTEN expression may lead to abnormally high expression of AKT2, which is related to the genesis and progression of colon cancer.