PCR检测慢性根尖周炎临床标本中牙髓卟啉单胞菌

Detection of porphyromonas endodontalis in chronic periapical periodontitis by PCR

目的:检测慢性根尖周炎临床标本中牙髓卟啉单胞菌(Porphyromonas endodontalis,Pe),以反映Pe在慢性根尖周炎中的存在情况。方法:采用聚合酶链反应(PCR)检测100例慢性根尖周炎临床标本中Pe16S rDNA,电泳,照相后计算其检出率。结果:PCR检测慢性根尖周炎临床标本中Pe的检出率为50%。结论:Pe较高的检出率提示Pe可能与根管感染密切相关。

Objective：To evaluate the prevalence of Pe in clinical samples of chronic periapical periodontitis.Methods：A total of 100 clinical samples of chronic periapical periodontitis were collected and Pe 16S rDNA was detected by using 16S rDNA gene-directed PCR.The detection rate of Pe were calculated.Results：The detection rate of Pe was 50% in the 100 clinical samples.Conclusion：The results indicate that Pe is closely associated with endodontic infections.