破骨细胞分化因子和破骨细胞生长抑制因子在牙周组织细胞中的表达

Expressions of ODF and OCIF in human periodontal ligament cells and human gingival fibroblasts

目的:检测炎症前细胞因子IL-1β和骨成熟因子PGE2对人牙周膜细胞和牙龈成纤维细胞破骨细胞分化因子(osteoclastdifferentiation factor,ODF)和破骨细胞生长抑制因子(osteoclastogenesis inhibitory factor,OCIF)表达的影响。方法:用RT-PCR和Southern-blot对该2种细胞在IL-1β或PGE2作用下细胞中ODF和OCIF mRNA的表达进行半定量。结果:上述2种细胞在IL-1β或PGE2作用下,ODF和OCIF的合成均有升高,各细胞合成ODF和OCIF的峰值随IL-1β或PGE2作用时间和浓度的不同而发生变化。结论:牙周组织中ODF和OCIF可能同时参与炎症反应,并受致炎因子IL-1β和骨代谢因子PGE2调节。

Objective：To detect the effects of IL-1β and osteotropic factor PGE2 on expressions of ODF and OCIF in cultured human periodontal ligament cells（HPDLC） and human gingival fibroblasts（HGF）.Methods：The expressions of ODF or OCIF mRNA were detected by RT-PCR and Southern-blot methods.Results：The results showed that IL-1β or PGE2 increased ODF and OCIF mRNA levels in both HPDLC and HGF cultures in dose-and time-dependent manner.Conclusion：The findings might suggest that the bone resorption during periodontitis might be regulated by ODF and OCIF,which are synthesized by periodontal tissue cells affected by proinflammatory cytokines,such as IL-1β and bone resorption cytokines,such as PGE2.