芪楮复筋方对大鼠坐骨神经损伤后再生和修复的作用

Effects of Qichu Fujin Recipe on regeneration and repair of injured sciatic nerve in rats

目的:探讨芪楮复筋方对大鼠坐骨神经损伤后的修复作用。方法:45只大鼠采用钳夹左侧坐骨神经法建立坐骨神经损伤模型,术后随机分为模型组、甲钴胺组和芪楮复筋方组。另15只正常大鼠作为正常对照组。甲钴胺组和芪楮复筋方组分别给予甲钴胺溶液[150 μg/(kg·d)]和芪楮复筋方煎液[35.2 g/(kg·d)]灌胃,模型组给予等量生理盐水灌胃。分别于术后第1、2和4周检测各组大鼠坐骨神经功能指数(sciatic function index,SFI)、腓肠肌湿质量残存率和腓肠肌肌细胞直径,免疫组织化学法检测神经组织S-100表达,电子显微镜观察神经超徽结构的改变。结果:术后第4周,甲钴胺组和芪楮复筋方组SFI、腓肠肌湿质量残存率、肌细胞直径、S-100阳性表达率、有髓神经纤维髓鞘厚度及轴突直径与正常对照组比较,差异有统计学意义(P<0.05.P<0.01),且均优于模型组(P<0.05,P<0.01)。除腓肠肌湿质量残存率和肌细胞直径外,甲钴胺组和芪楮复筋方组各指标间比较差异无统计学意义(P>0.05)。结论:芪楮复筋方可促进周围神经损伤后神经的再生和功能恢复,延缓靶器官肌肉萎缩。

Objective： To explore the effects of Qichu Fujin Recipe （QCFJR）, a compound traditional Chinese medicine, in repairing sciatic nerve injury in rats. Methods： A total of 60 male Sprague-Dawley （SD） rats were randomly divided into four groups： normal control group, untreated group, mecobalamin group and QCFJR group. Except the normal control group, sciatic nerve injury was induced by crushing of left sciatic nerve. Rats in the mecobalamin group were intragastrically administered with mecobalamin solutions 150 μg/（kg·d）, and rats in the QCFJR group were intragastrically administered with QCFJR 35.2 g/（kg·d）, while rats in the untreated group were intragastrically administered with normal saline （0.5 mL/d）, once a day for 4 weeks respectively. Sciatic function index （SFI） was determined by walking tract analysis 1 week and 2 and 4 weeks after crushing. Five rats in each group were sacrificed for histological observation at the different time points. The remnant rate of gastrocnemius wet weight and the diameter of gastrocnemius cells were calculated. Expression of S-100 protein in the distal stump of injured nerve was observed by using immunohistochemical method. Distal injured sciatic nerves were determined with toluidine blue staining and observed under a light microscope 1 week and 2 and 4 weeks after operation. Diameter of axon and depth of myelin sheath were calculated by an image analysis system. Ultrastructure of nerve fibers was determined with uranyl acetate and lead citrate staining and observed under an electron microscope. Results： Compared with the normal control group, SFI, remnant rate of gastrocnemius wet weight, diameter of gastrocnemius cells, expression of S-100, diameter of axon and depth of myelin sheath in the mecobalamin group and the QCFJR group were significantly decreased at different time points （P〈0.05）, superior to those of the untreated group （P〈0.05, P〈0.01）. Except the remnant rate of gastrocnemius wet weight and the diameter of gastrocnemius cells, there were no significant differences in other indexes between the mecobalamin group and the QCFJR group 1 week and 2 and 4 weeks after crushing （P〉0.05）. Conclusion： QCFJR can stimulate the regeneration and repair of nerve fiber and delay the skeletal muscle atrophy after sciatic nerve injury in rats.