期刊文献+

Combined Use of RGD-peptide Modified PLGA and TGF-β1 Gene Transfected MSCs to Improve Cell Biobehaviors in vitro

Combined Use of RGD-peptide Modified PLGA and TGF-β1 Gene Transfected MSCs to Improve Cell Biobehaviors in vitro
下载PDF
导出
摘要 In order to improve the surface properties of PLGA polymer for a better material/cell interface to modulate the cells behaviors, we prepared a novel three-block copolymer, PLGA-[ASP-PEG], and immobilized an RGD-containing peptide, Gly-Arg-Gly-Asp-Ser-Pro-Cys (GRGDSPC) on the surface of it. Transforming growth factor-β1 (TGF-β1) was transfected into bone marrow stromal cells (MSCs) employed as seeded cells. Cell adhesion, spreading, proliferation and differentiation on this material were investigated. The results showed that the cell adhesive ratio on RGD-modified materials was higher than on un-modified materials (P〈0.05). The extent of cell spreading was also wider on RGD-modified materials than on un-modified materials. Cell proliferation indices of transfected MSCs were increased as compared with the un-transfected MSCs (P〈0.05). The ALP activities in the MSCs cultured with RGD-modified materials were higher than on un-modified materials after 14 days (P〈0.05), and those in transfected MSCs were higher than in un-transfected MSCs (P〈0.05). It was suggested that the combined use of RGD-modification and TGF-β gene transfection could improve the interaction of biomaterial and cells. In order to improve the surface properties of PLGA polymer for a better material/cell interface to modulate the cells behaviors, we prepared a novel three-block copolymer, PLGA-[ASP-PEG], and immobilized an RGD-containing peptide, Gly-Arg-Gly-Asp-Ser-Pro-Cys (GRGDSPC) on the surface of it. Transforming growth factor-β1 (TGF-β1) was transfected into bone marrow stromal cells (MSCs) employed as seeded cells. Cell adhesion, spreading, proliferation and differentiation on this material were investigated. The results showed that the cell adhesive ratio on RGD-modified materials was higher than on un-modified materials (P〈0.05). The extent of cell spreading was also wider on RGD-modified materials than on un-modified materials. Cell proliferation indices of transfected MSCs were increased as compared with the un-transfected MSCs (P〈0.05). The ALP activities in the MSCs cultured with RGD-modified materials were higher than on un-modified materials after 14 days (P〈0.05), and those in transfected MSCs were higher than in un-transfected MSCs (P〈0.05). It was suggested that the combined use of RGD-modification and TGF-β gene transfection could improve the interaction of biomaterial and cells.
出处 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2009年第5期592-598,共7页 华中科技大学学报(医学英德文版)
关键词 tissue engineering biomaterial PLGA RGD transforming growth factor β bone marrow stromal cells tissue engineering biomaterial PLGA RGD transforming growth factor β bone marrow stromal cells
  • 相关文献

参考文献11

  • 1郭晓东.Surface Modification of Biomimetic PLGA-(ASP-PEG) Matrix with RGD-Containing Peptide:a New Non-Viral Vector for Gene Transfer and Tissue Engineering[J].Journal of Wuhan University of Technology(Materials Science),2006,21(3):41-43. 被引量:3
  • 2Zhao J,Quan D,Liao K,et al.PLGA- (L-Asp-alt-diol) (x)-PLGAs with different contents of pendant amino groups[].Macromol Biosci.2005
  • 3Langer R,Vacanti JP.Tissue engineering[].Science.1993
  • 4Bonassar L J,Vacanti C A.Tissue engineering: the first decade and beyond[].Journal Of Cellular Biochemistry Supplement.1998
  • 5Quirk R A,Chan W C,Davies M C,et al.Poly(L-lysine)-GRGDS as a biomimetic surface modifier for poly (lactic acid)[].Biomaterials.2001
  • 6Hersel U,Dahmen C,Kessler H.RGD modified polymers: biomaterials for stimulated cell adhesion and beyond[].Biomaterials.2003
  • 7Lebaron R G,Athanasiou K A.Extracellular matrix cell adhesion peptides: functional applications in orthopedic materials[].Tissue Engineering.2000
  • 8M. Wiedmann-AL-Ahmad,R. Gutwald,G. Lauer et al.How to optimize seeding and culturing of human osteoblast-like cells on various biomaterials[].Biomaterials.2002
  • 9Jaiswal N,Haynesworth SE,Caplan AI,et al.Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro[].Journal of Cellular Biochemistry.1997
  • 10Yang XB,Roach HI,Clarke NM,et al.Human osteoprogenitor growth and differentiation on synthetic biodegradable structures after surface modification[].Bone.2001

二级参考文献7

  • 1Collins L;Gustafsson K;Fabre J.Tissue-binding Properties of a Synthetic Peptide DNA Vector Targeted to Cell Membrane Integrins:a Possible Universal Nonviral Vector for Organ and Tissue Transplantation[J],2000(06).
  • 2Bianco P;Robey P.Stem Cells in Tissue Engineering,2001(6859).
  • 3Nasseri B;Ogawa K;Vacanti J.Tissue Engineering:An Evolving 21st-century Science to Provide Biologic Replacement for Reconstruction and Transplantation,2001(05).
  • 4Shewring L;Collins L;Lightmao SL.The Non-viral Vector System for Efficient Gene Transfering to Corneal Cell via Membrane Integrin,1997.
  • 5Louise C;Kenth G;John W.Tissue-binding Properties of a Synthetic Peptide DNA Vector Targeted to Cell Membrane Integrins,2000(06).
  • 6Ma H;Diamond S.Nonviral Gene Therapy and Its Delivery Systems[J],2001(01).
  • 7Richardson T;Murphy W;Mooney D.Polymeric Delivery of Proteins and Plasmid DNA for Tissue Engineering and Gene Therapy[J],2001(1-3).

共引文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部