海狗油脂肪乳对大鼠的一般生殖毒性研究

Toxic Effects of Seal Oil Emulsion on General Reproduction in Rats

背景与目的:观察海狗油脂肪乳对大鼠配子成熟、交配行为、生育力和早期胚胎发育方面的毒性作用。材料与方法:160只Wistar大鼠随机分为海狗油脂肪乳不同剂量组(250、500和1000mg/kg)和溶剂对照组,共4组。雄性大鼠在交配前连续腹腔注射海狗油脂肪乳35d,雌性大鼠在交配前连续腹腔注射给予海狗油脂肪乳18d,相同剂量组内大鼠按雄:雌=1:1同笼交配,连续10d。在确定雌鼠受精后处死同笼的雄鼠,解剖检查睾丸和附睾等生殖器官及精子的活力。雌鼠交配期间继续给药至妊娠第14d处死,解剖检查卵巢、子宫和胚胎的情况。结果:给药期间各剂量组大鼠除体重增长缓慢(体重增重平均最大差值达到50g,与对照组比较,差异显著,P<0.05)外,精神、行为、活动及被毛正常;雄性大鼠生殖器官发育良好,性功能正常,精子数量多、成熟,畸形率低。妊娠大鼠500mg/kg和1000mg/kg组的活胎数减少(窝平均最大差值2.0个),胎仔平均体重降低(每只平均最大差值0.03g)以及1000mg/kg剂量组的交配率为65%(13/20),与对照组比较差异有统计学意义(P<0.05)。妊娠率、黄体数、着床数、吸收胎数、死胎数、受精卵着床前后丢失率和胎仔体表畸形数等与对照组比较无显著差异(P>0.05)。结论:海狗油脂肪乳可影响成年大鼠体重增长,引起妊娠大鼠早期胚胎和胎仔的生长发育障碍,但对大鼠其他生殖功能无明显损害作用。

BACKGROUND AND AIM：To observe the toxic effect of seal oil emulsion on the ability of maturity,mating,fertility and early fetal growth and development.MATERIALS AND METHODS：Wistar rats were randomly allocated into 0,250,500 and 1 000 mg/kg dose groups,and given seal oil emulsion orally for 5 weeks in male rats,2 weeks in female rats respectively before mated,and then mated for 10 days.When the female rats were confirmed pregnant,the male rats were executed and their testicle,epididymis and sperm were examined.The seal oil emulsion treatment continued until 14th day of pregnancy and the ovary, uterus and embryo of the female rats were studied. RESULTS： The treatment groups were normal in spirit, behavior, activity and hair quality but the body weight gain was slow （average most value 50 g,P 〈 0.05）. The genitalia development, sexual function, the quantity and quality of sperm were normal. The number of live embryo （litter average 2.0） , the fetal body weight （average 0.03 g）, and the mating ratio in 1 000 mg/kg group was 65% （P 〈0.05） .There were no significant differences in the pregnancy rate, the number of corpus luteum, implantation, absorbed embryo and dead embryo, the rate of lost oosperm and fetus abnormality between the treatment and the control groups （P 〉 0.05）. CONCLUSION： Within the range of experiment doses, seal oil emulsion obviously decreased body weight gain of male rats, impaired embryonic and fetal development but did not effect the development of genitalia, mating behavior, sperm and ovum maturation and live fetus.