纯钛表面改性对细菌粘附的影响

Influence of modification on Ti on adhesion of bacteria

目的:研究氮离子溅射和氮等离子浸没注入对纯钛表面细菌粘附能力的影响。方法:制作纯钛试件288件,各随机选出96件,分别采用氮离子溅射和氮等离子浸没注入对其表面改性,纯钛组为对照组,氮离子溅射组为实验1组,氮等离子浸没注入组为实验2组。在实验1、2组和对照组试件表面粘附血型链球菌、粘性放线菌、白色念珠菌和金黄色葡萄球菌,分别进行细菌体外粘附实验。用菌落形成单位计数法统计分析氮离子溅射和氮等离子浸没注入对各种细菌粘附量的影响。结果:在细菌粘附24h、48h、168h后,上述4种细菌在氮离子溅射组和氮等离子浸没注入组表面粘附量较对照组表面粘附量显著减少(P<0.001),其中4种细菌在氮等离子浸没注入组表面粘附量明显少于氮离子溅射组(P<0.001)。结论:纯钛表面氮离子溅射和氮等离子浸没注入均可抑制细菌粘附,氮等离子浸没注入较氮离子溅射抑制细菌粘附效果更明显。

Objective： To study the influence of N＋ ion sputtering and N＋ plasma ion implantation on clinically used Ti on adhesion of bacteria. Method： Two hundred and eighty-eight pieces of Ti were made, and randomly chosen ninety- six of them, and then TiN coating on the surface of them by N＋ ion sputtering and N＋ plasma ion implantation technique saved as experimental group 1 and group 2, while those of non-coated, as control. Definite amounts of Strep.sangnis, Actino- myces viscosus, Candida albicans and Staphylococcus aureus were adhered onto the surfaces of TA2, and then the number of bacteria was assayed by means of clone forming unit （CFU）method at adhesion period 24 h.48 h. 168 h respectively. Re- suit： At each period, the number of the adhered Strep.sanguis, Actinomyces viscosus, Candida albicans and Staphylococcus aureus was significantly reduced in group 1 and group 2 （P 〈 0.001）, and the number of the adhered Strep.sanguis, Actino- myces viscosus, Candida albicans and Staphylococcus aureus was less significantly in group 2 than group 1. Conclusion. N＋ ion sputtering and N＋ plasma ion implantation on clinically used Ti can reduce bacterium adhesion on Ti, and N＋ plasma ion implantation can reduce more significantly bacterium adhesion on Ti than N＋ ion sputtering .