丙酸氟替卡松及左卡巴斯汀对实验性变应性鼻炎大鼠水通道蛋白5的影响

Effect of intra nasally applied fluticasone propionate and levocabastine on the expression of aquaporin 5 in nasal mucosa of rat with experimental allergic rhinitis

目的:观察鼻腔局部应用丙酸氟替卡松和左卡巴斯汀对实验性变应性鼻炎(AR)大鼠水通道蛋白5(AQP5)表达的影响。方法:40只Wister大鼠随机分为AR组30只和对照组10只。AR组建模后,随机均分为3组:AR+丙酸氟替卡松组(F组)、AR+左卡巴斯汀组(L组)和AR组(10只)。3组大鼠治疗28 d后,应用免疫组织化学检测AQP5在各组中的表达。结果:免疫组织化学显示AQP5在各组分布相同,统计学分析显示F组与AR组、L组比较,差异有统计学意义(P<0.05),但L组与AR组比较,差异无统计学意义(P>0.05);L组与对照组比较,差异有统计学意义(P<0.05)。结论:AQP5在实验性AR大鼠鼻黏膜的强表达,提示AQP5参与了AR的发病过程,与腺体过度分泌、组织水肿有关。糖皮质激素可以明显下调AQP5的表达量,H1受体阻滞剂对AQP5的表达量无明显影响。

Objective：To investigate the expression and distribution of aquaporin 5（AQP5） in allergic rhinitis（AR）treated by fluticasone propionate and levocabastine.Method：Forty Wister rats were divided randomly into AR（n=30） and control groups（n=10）.After AR models were established,the AR rats were divided evenly into F group,L group and AR control group.Three groups were treated respectively for 28 days,then the expression of AQP5 in nasal mucous membrane were detected by immumohistochemistry assay.Result：The distribution of AQP5 was consistent in all groups.The expression of AQP5 in F group was significantly different from L group and AR group（P 〈 0.05）,while there was no significant difference between that of AR group and L group（P〉0.05）.The expression of AQP5 in L group was significantly different from that in control group（P〈0.05）.Conclusion：High expressions of AQP5 in rat with AR indicated the positive correlation between AQP5 and AR.AQP5 might be one of pathological factors of AR concered with gland＇s excessive secretion and tissue edema.Glucocorticoid can down-regulate the expression of AQP5,but H1-receptor antagonist can not reduce the expression of AQP5.